Publications by authors named "Aparajitha Srinivasan"
Unlabelled: The industrial amino acid production workhorse, Corynebacterium glutamicum naturally produces low levels of 2,3,5,6-tetramethylpyrazine (TMP), a valuable flavor, fragrance, and commodity chemical. Here, we demonstrate TMP production (∼0.8 g L-1) in C.
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- * Pseudomonas putida has been identified as a promising microorganism for producing isoprenol due to its ability to utilize low-cost plant biomass.
- * Researchers have successfully engineered P. putida using computational models to optimize the pathway for isoprenol production, achieving a titer of 3.5 g/L, which highlights the potential for sustainable biofuel production from renewable sources.
Vitamin E is a dietary supplement synthesized only by photosynthetic organisms and, hence, is an essential vitamin for human well-being. Because of the ever-increasing demand for natural vitamin E and limitations in existing synthesis modes, attempts to improve its yield using plant cultures have gained traction in recent years. With inflating industrial production costs, integrative approaches to conventional bioprocess optimization is the need of the hour for multifold vitamin E productivity enhancement.
View Article and Find Full Text PDFGenomics Characterization of an Engineered in Bioreactor Cultivation Under Ionic Liquid Stress.
Front Bioeng Biotechnol
November 2021
Article Synopsis
- The study focuses on a genetically engineered microbial strain (BRC-JBEI 1.1.2) that efficiently produces bioproducts such as amino acids and biofuels.
- Researchers resequenced the strain and analyzed how gene expression changes when transitioning from shake flasks to bioreactors, especially when exposed to an ionic liquid called cholinium lysinate.
- Findings revealed distinct metabolic and regulatory responses essential for growth in industrial settings, along with variations in the accumulation of organic acids and bioproducts, enhancing our understanding of the engineered strain's performance in bioproduction.
Anti-drug antibody (ADA) formation is a major complication in treatment of the X-linked bleeding disorder haemophilia B (deficiency in coagulation factor IX, FIX). Current clinical immune tolerance protocols are often not effective due to complications such as anaphylactic reactions against FIX. Plant-based oral tolerance induction may address this problem, as illustrated by the recent first regulatory approval of orally delivered plant cells to treat peanut allergy.
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