A type of self-assembled and label-free DNA-modified electrochemical biosensors based on magnetic α-FeO/FeO heterogeneous nanorods for ultra-sensitive detection of CYP2C19*3.

CYP2C19*3 enzyme plays a pivotal role in drug metabolism and is tightly regulated by the CYP2C19*3 gene. Therefore, quantification of CYP2C19*3 gene holds paramount importance for achieving personalized medication guidance in precision medicine. In this project, the magnetic electrochemical biosensors were constructed for the ultra-sensitive detection of CYP2C19*3 gene.

Nucleic acid aptasensor with magnetically induced self-assembly for the detection of EpCAM glycoprotein.

A "turn-on" aptasensor for label-free and cell-free EpCAM detection was constructed by employing magnetic α-FeO/FeO@Au nanocomposites as a matrix for signal amplification and double-stranded complex (SH-DNA/Apt probes) immobilization through Au-S binding. α-FeO/FeO@Au could be efficiently assembled into uniform and stable self-assembly films via magnetic-induced self-assembly technique on a magnetic glassy carbon electrode (MGCE). The effectiveness of the platform for EpCAM detection was confirmed through differential pulse voltammetry (DPV).

Electrochemical peptide nucleic acid functionalized α-FeO/FeO nanosheets for detection of CYP2C19*2 gene.

The CYP2C19*2 gene carriers and non-carriers are closely related to the dosage of clopidogrel. To correctly guide the use of clopidogrel and promote individualized therapy, an ultra-sensitive electrochemical biosensor was developed for the detection of CYP2C19*2 gene. The heterogeneous α-FeO/FeO nanosheets were prepared via the hydrothermal-calcination process, and the preparation parameters were optimized.

Symmetry dual functional pyrimidine-BODIPY probes for imaging targeting and activity study.

Nondestructive diagnosis of tumor has always been the goal of scientists. Fluorescent dyes have become the rising star in the field of cancer diagnosis because of their excellent characteristics. Therefore, in this work, fluorescence probes and with anti-tumor activity were constructed by introducing pyrimidine groups with high anti-tumor activity using fluorescence dye BODIPY as parent nucleus.

Label-free electrochemical aptasensor based on magnetic α-FeO/FeO heterogeneous hollow nanorods for the detection of cancer antigen 125.

A label-free electrochemical aptasensor based on magnetic α-FeO/FeO heterogeneous hollow nanorods was developed for the detection of cancer antigen 125 (CA125). Magnetic α-FeO/FeO heterogeneous hollow nanorods were successfully prepared by the hydrolysis-calcination method, functionalized with polyethyleneimine (PEI), and modified with HAuCl to form magnetic α-FeO/FeO-Au nanocomposites with a layer of 5 nm gold nanoparticles (Au NPs) on the surface. The magnetic α-FeO/FeO-Au nanocomposites were used to fix the DNA-aptamer probe to amplify the current signal.

Nano-Graphene Enclosed Multi Nitrogen: Dynamic Hierarchical Self-Assemble Property for Lithium Ion Storage.

Led to significant capacity improvement to 1800 mA/h after 100 cycles for nano-graphene-N₄, which is the first report for a carbonaceous materials anode. In addition, the doping level, id est, number of nitrogen atoms, had a significant influence on the molecular self-assembled structures through hierarchical self-assembly. As the nitrogen concentration increased, the -space between the nanosheets increased from 3.

A Novel Process for the Preparation of ZnO Nanoparticles and ZnO/NiZnFe₂O₄ Nanocomposites.

A novel solution combustion and gel calcination process for the preparation of ZnO nanoparticles and ZnO/NiZnFe₂O₄ nanocomposites was introduced. The phase identification, morphologies and magnetic properties of ZnO nanoparticles and ZnO/NiZnFe₂O₄ nanocomposites were confirmed by X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscope (TEM), vibrating sample magnetometer (VSM). The results showed that the concentration of zinc nitrate and the calcination temperature were two key factors for the grain size, crystallinity and properties of ZnO nanoparticles, while, the mass ratio of ZnO and NiZnFe₂O₄ in ZnO/NiZnFe₂O₄ nanocomposites was the important factor for the specific magnetizations () of ZnO/NiZnFe₂O₄ nanocomposites.

Klotho Inhibits Unilateral Ureteral Obstruction-Induced Endothelial-to-Mesenchymal Transition TGF-β1/Smad2/Snail1 Signaling in Mice.

This study aimed to evaluate repression of Klotho on unilateral ureteral obstruction (UUO)-induced renal fibrosis and endothelial-to-mesenchymal transition (EndoMT) in mice. Renal fibrosis model was established by UUO in C57BL/6 male mice. Recombinant Klotho protein was administered to UUO mice as treatment group, and the mice in sham and UUO group were administered with an equal volume of vehicle.

Adsorption Performance of Congo Red onto Magnetic NiCoZnFe₂O₄ Nanoparticles Prepared via the Rapid Combustion Process.

Magnetic NiCoZnFe₂O₄ nanoparticles were prepared via the rapid combustion process, the morphology and magnetic property of them were investigated by SEM, TEM, XRD, VSM, and FTIR. The as-prepared magnetic NiCoZnFe₂O₄ nanoparticles calcined at 400 °C for 2 h with absolute alcohol of 20 mL were characterized with the average nanoparticle size of around 25 nm and the specific magnetization of 35.2 emu/g.

1,25(OH)D Activates Autophagy to Protect against Oxidative Damage of INS-1 Pancreatic Beta Cells.

Diabetes mellitus is a serious disease endangering human health worldwide. Vitamin D (Vit D) is a well-characterized regulator of calcium-phosphorus metabolism that also exerts other biological effects extending far beyond mineral homeostasis. Some epidemiological studies have suggested that Vit D has a role in defense against diabetes, although the mechanism remains unclear.

Dynamic Hierarchical Self-Assemble Small Molecule Structure Hexabenzocoronene for the High-Performance Anodes Lithium Ion Storage.

This study examined the characteristics of small molecular structure nano-graphene in a dynamic hierarchical self-assembly and found that graphene is rearranged under its own pressure during dynamic aggregation and water ripples are formed by the d-spacing. The composition and structure were studied using a range of material characterization techniques. No covalent bonds were observed between molecules, and the self-assembled driving force was the only intermolecular interaction: Van der Waals' force in the intra-layer and π-π interactions between layers.

Ginsenoside-Rg1 Protects against Renal Fibrosis by Regulating the Klotho/TGF-β1/Smad Signaling Pathway in Rats with Obstructive Nephropathy.

Ginsenoside-Rg1 (G-Rg1) is an agent isolated from Panax ginseng that exerts anti-fibrotic effects; however, the mechanism is still unclear. Herein, we investigated whether G-Rg1 administration can mitigate or reverse unilateral ureteral obstruction (UUO)-induced renal fibrosis by regulating the Klotho/transforming growth factor (TGF)-β1/Smad signaling pathway in rats. Sprague-Dawley male rats were subjected to UUO, and rats in the treatment group were administered G-Rg1 or G-Rg1 plus Klotho short hairpin RNA interference (shRNA), while rats in the control and model groups were administered vehicle for 14 d.

Adsorption Kinetics of Bovine Serum Albumin onto NiZnFe₂O₄/SiO₂ Nanocomposites Prepared via the Solution Combustion Process.

Magnetic Ni0.5Zn0.5Fe2O4/SiO2 nanocomposites were prepared via the solution combustion process, their structure and magnetic properties were investigated by XRD, SEM, TEM and VSM.

Adsorption Performance of DNA onto Magnetic Core-Shell Structure (1-)NiZnFe₂O₄/SiO₂ Nanopowders Prepared via Methanol Combustion Process.

Magnetic core-shell structure (1-x)Ni0.5Zn0.5Fe2O4/xSiO2 (x = 0-0.

Plasma s-Klotho is related to kidney function and predicts adverse renal outcomes in patients with advanced chronic kidney disease.

To investigate whether the soluble Klotho (s-Klotho) level in patients with chronic kidney disease (CKD) is related to kidney function and whether a low s-Klotho level can predict adverse renal outcomes or CKD progression in patients with advanced CKD. 112 patients with CKD stages 3-5 and 30 healthy volunteers were enrolled. Blood samples were collected to measure serum creatinine, calcium, phosphorus, intact parathyroid hormone, and hemoglobin.

Cyclosporine A Suppresses the Activation of the Th17 Cells in Patients with Primary Sjögren's Syndrome.

Primary Sjögren's syndrome (pSS) is a common autoimmune disease involving abnormal Th17 activation. The aim of the current study was to investigate the immunosuppression effect of Cyclosporine A (Cys A), a potent immunosuppressor on the proliferation and activation of T cells, on the activation of Th17 cells. Blood samples from both inactive and active pSS patients as well as healthy controls were collected and serum and peripheral blood mononuclear cells (PBMCs) were collected and tested for IL-17 and RORγt expression.

Tranilast attenuates TGF-β1-induced epithelial-mesenchymal transition in the NRK-52E cells.

We previously reported that tranilast can halt the pathogenesis of chronic cyclosporine nephrotoxicity in rats via the transforming growth factor-β (TGF-β) /Smad pathway, an important signaling system involved in epithelial-mesenchymal transition (EMT), but the exact underlying cellular mechanisms are not yet clear. Thus, by selecting TGF-β1-induced normal rat kidney proximal tubular epithelial cells (NRK-52E) as a model, we demonstrated potential modifying effect of tranilast on EMT-induced by TGF-β1 in vitro. NRK-52E cells were incubated with the blank vehicle (Dulbecco's modified Eagle's medium and F-12 (DMEM/F12) added with 10% fetal bovine serum (FBS)), 10 ng/ml TGF-β1 alone or together with 100, 200 or 400μM tranilast for 48 h after incubation in medium containing 1% FBS for 24 h.

[Research on the effects of PIAS3 expression on the invasion of glioma TJ905 cells].

Objectives: To investigate the function and possible mechanisms of PIAS3 expression on the invasion of TJ905 cells.

Methods: PIAS3 overexpression vectors were constructed and PIAS3 siRNA were chemically synthesized, which were separately transfected into TJ905 cells for upregulation or downregulation of PIAS3 expression levels in TJ905 cells. After that, the invasive effects of TJ905 cells were measured by Transwell assay, and the expression of PIAS3, tissue inhibitor of metalloproteinases (TIMP)3, matrix metalloprotease (MMP)-2, and MMP-9 were identified by Western blot.