Do lipases and amylases of an endangered Indian carp Hypselobarbus pulchellus play a role in visceral fat dynamics; evidence of in vitro macromolecular interactions.

Fat depots or triglycerides are hydrolysed by the action of lipases in fish to be used for energy and/or for growth and reproduction. In herbivores fishes, de novo synthesis of lipids from non- lipid substrates (glucose) leads to fat deposits and/or fatty infiltration in organs especially on ovaries limiting its normal functions. This study was aimed to understand lipases from the digestive tract (DT) of adult Hypselobarbus pulchellus of different sizes, their partial purification, characterisation and their isozymes.

Potential utility of callus proteases as a milk clotting alternative to naturally propagated proteases.

This study was intended to understand suitability of calli proteases (CCE) as vegetable coagulant through delineating their milk clotting potential and casein (whole and κ-casein) hydrolysis pattern in comparison to their plant stem crude enzyme (PSCE), rennin and Enzeco (positive controls). Stem calli was induced using two concentrations of naphthalene acetic acid (NAA) [0.5 mg/L (M1), 1 mg/L (M2)] with fixed concentration (1 mg/L) of 6-benzylaminopurine (BAP).

Purification, characterization and fibrino(geno)lytic activity of cysteine protease from latex.

Protease was isolated and purified from latex and its hemostatic potential was analyzed. Crude latex enzyme was purified through ion exchange and gel filtration chromatography. Purified protease was characterized and its thrombin-like (coagulant assay, fibrinogen polymerizing, and fibrinogenolytic activity) and plasmin-like (blood and plasma clot lysis) activities were assessed accordingly.

Three phase partitioning to concentrate milk clotting proteases from Wrightia tinctoria R. Br and its characterization.

Wrightia tinctoria stem proteases were partially purified for the first time through a non-chromatographic technique, three phase partitioning (TPP), to concentrate the milk clotting proteases. Various parameters like salt and solvent concentration that affect the partitioning of the protease were examined. Maximum recovery and purification fold of the protease activity were found in the interfacial phase (IP) with 60% ammonium sulphate and 1:1 crude enzyme to t-butanol.