Molecular probe to visualize the effect of a glycolytic inhibitor on reducing NADH levels in a cellular system.

The reduced form of nicotinamide adenine dinucleotide, commonly known as NADH, is an essential coenzyme existing in living organisms. Due to its involvement in various biological process, fluorescence imaging of intracellular NADH levels in different pathological conditions has emerged as an interesting area of research. We report here the exploration of a fluorescent probe, MQ-CN-BTZ, as a dual-channel NADH imaging agent (green and red channels) for cellular systems.

Advancements in Three-Dimensional Super-Resolution Ultrasound Imaging: A Narrative Review.

The lack of sensibility of traditional ultrasound (US) imaging to the slow blood flow in small vessels resulted in the development of microbubble (MB) contrast agents. These MBs are given intravenously, and US imaging can detect them quite effectively. This noninvasive imaging method, known as contrast-enhanced US (CEUS), now makes it possible to accurately assess tissue perfusion and blood flow.

Benign mid-gap halide vacancy states in 2D-bismuth-based halide perovskite microcrystals for enhanced broadband photodetectors.

Lead halide perovskites are widely recognized for their exceptional defect tolerance, setting the benchmark for high-performance optoelectronic applications. Conversely, low-toxicity perovskite-inspired materials (PIMs) typically exhibit suboptimal optoelectronic performance, primarily due to their intrinsic susceptibility to defects. In this study, we address this limitation by exploring the effects of halide vacancies in PIMs through the synthesis of non-stoichiometric CsBiBrI microcrystals (MCs) with a trigonal crystal structure, incorporating iodine vacancies.

Exploring the potential anti-senescence effects of soybean-derived peptide Soymetide in mice hippocampal neurons via the Wnt/β-catenin pathway.

Soybean-based foods enhance cognitive functions by influencing hippocampal mechanisms. These salutary effects have so far been attributed to isoflavones present in soybeans. Considering cellular senescence contributes to cognitive decline and that no specific soy-derived peptides are known for their potential to mitigate senescence, we examined the efficacy of a thirteen amino acid soy-derived peptide, Soymetide, on a doxorubicin-induced senescence mice model.

Mechanistic investigation on cellular internalization triggering structure-induced conformational modulation of boron-nitrogen luminogens.

Exploring the effects of electron donor (D) and acceptor (A) functional groups in tuning the condensed state properties has been a challenging yet efficient approach to reveal promising materials for cutting-edge applications. Herein, a series of boron-nitrogen (BN) incorporated organic congeners (NBNMe2, NBOMe, NBF, NBCl, NBBr, NBCN, NBPy) appended with functional groups having various degrees of D/A characteristics were developed and their potential in controlling supramolecular assembly and condensed state luminescence features (>90 nm redshift in ) was explored. Despite the minor structural engineering in BN-based small molecules, they effectively modulated conformational orientation and molecular packing, leading to the directed growth of distinct and highly ordered self-assembly patterns, , nanosheets, nanospheres, nanowires, and nanorods.

Longitudinal Outcomes in Pi*MZ Alpha-1 Antitrypsin Deficient Individuals with Tobacco Smoking History from the SPIROMICS Cohort.

Background: Reliable data about the natural history of lung function decline in alpha-1 antitrypsin (AAT) deficient Pi*MZ heterozygotes is largely missing. We hypothesized that, in adults with a tobacco smoking history, lung function deteriorates faster in Pi*MZ compared to Pi*MM genotype.

Methods: We identified 1856 Pi*MM and 79 Pi*MZ participants with ≥20 pack-years tobacco smoking history from the SPIROMICS cohort by DNA sequencing and followed them over a median of 4.

Fabrication of Folic Acid-Derived Carbon Dot-Conjugated Chitosan Nanospheres as Theragnostic Agents for pH-Responsive Anticancer Drug Delivery.

The favorable success rate in cancer treatment predominantly depends on precise diagnosis with target-specific drug delivery, which can regulate the patient survival outcome rate. Moreover, proper tracking of the system's pH is very much crucial as most of the therapeutic's action and release rate depend on it. Therefore, this work has been intended to fabricate a folic acid-derived carbon dot (FACD) decorated with chitosan (Cs) in order to form nanospheres (FACD-Cs-Ns) for anticancer doxorubicin hydrochloride (Dox.

Exposures from oil and gas development and childhood leukemia risk in Colorado: A population-based case-control study.

Background: Children living in upstream oil and natural gas (O&G) areas may be exposed to leukemogens and at increased risk for acute lymphocytic leukemia (ALL).

Methods: We conducted a case-control study of children born in Colorado between 1992 and 2019. We matched 451 children diagnosed with ALL at age 2-9 years starting in 2002 to 2706 controls on birth month/year and Hispanic ethnicity.

Lipoid Proteinosis-An Unusual Cause of Hoarseness of Voice in a Child: Case Report.

Lipoid proteinosis is a rare hereditary multisystemic familial disorder characterised by intracellular deposition of hyaline material (Rao and Koppada in Indian J Dermatol Venereol Leprol, 2015; Shah and Shah in J Oral Maxillofac Pathol 26:236-241, 2022). The hallmark findings are pox like scarred skin lesion, hoarseness of voice and selective brain parenchymal calcification (Rao and Koppada in Indian J Dermatol Venereol Leprol, 2015). We present a case of biopsy proven lipoid proteinosis with typical radiological findings.

Examining the contribution of charge transport layers to boost the performance over 26% in SrPCl absorber-based bifacial perovskite solar cells.

Strontium phosphorus chloride (SrPCl) presents a promising option for photovoltaic (PV) applications due to its distinctive optical, electrical, and structural characteristics. This research uses density functional theory (DFT) to examine its structural stability and optoelectronic properties. The PV performance of SrPCl-based cell designs was examined, utilizing an electron transport layer (ETL) of ZnO and four different hole transport layers (HTLs): CuO, CBTS, MoO, and CuI.

Synergistic Therapeutic Effects of Zn ions and Pimozide in Breast Cancer Cells.

Zinc (Zn) ions play a crucial role in cancer therapy due to their ability to induce reactive oxygen species (ROS) generation, oxidative stress, and ferroptosis. Combining Zn ions with other therapeutic agents can significantly enhance their efficacy through synergistic mechanisms. This study explores the synergistic mechanism of Zn ions form pH-responsive ZIF-8 and repurposed drug Pimozide in tumor microenvironment mimic conditions.

Microbiological Profiles and Resistance Patterns in Pediatrics With Cancer: An 8-Year Study at a Comprehensive Cancer Center in Jordan.

Introduction: Infections impact morbidity and mortality in pediatric cancer patients, yet limited studies have assessed the microbiological profiles and susceptibility patterns of pathogenic bacteria in this population. This study aimed to investigate bacterial profiles and temporal resistance changes in pediatrics with cancer.

Methods: We identified positive cultures between January 2015 and December 2022 for pediatric patients diagnosed with cancer at age < 18 years.

LungGENIE: the lung gene-expression and network imputation engine.

Background: Few cohorts have study populations large enough to conduct molecular analysis of ex vivo lung tissue for genomic analyses. Transcriptome imputation is a non-invasive alternative with many potential applications. We present a novel transcriptome-imputation method called the Lung Gene Expression and Network Imputation Engine (LungGENIE) that uses principal components from blood gene-expression levels in a linear regression model to predict lung tissue-specific gene-expression.

Amphiphilic food polypeptides via moderate enzymatic hydrolysis of chickpea proteins: Bioprocessing, properties, and molecular mechanism.

Plant proteins are a promising source for producing amphiphilic polypeptides with tailored techno-functional properties to be used in various food applications, such as fat replacers. This study investigated the effects of moderate enzymatic hydrolysis on amphiphilic polypeptide generation, by understanding the relationship of bioprocess - protein structure - functionality - amphiphilicity mechanism. Compared to non-specific protease alcalase, the specific protease trypsin catalyzed the production of polypeptides with higher surface hydrophobicity and relatively high molecular weight.

Endostatin and Cystatin C as Predictors of 1 Month Renal Function Change in Patients With Left Ventricular Assist Device Support.

First-month renal function trajectories after left ventricular assist device (LVAD) implantation vary widely. Lack of renal function improvement (RFI) and postoperative acute kidney injury (AKI) are associated with increased mortality. This study evaluates plasma levels of endostatin and cystatin C as predictors of RFI and AKI 1 month post-LVAD implantation.

Modular Approach for Photoinduced Cycloaddition Enabling the Synthesis of Diverse Bioactive Oxazoles.

The synthesis of bioactive oxazoles is often inefficient, and the reported methods remain largely unexplored for complex derivatives. To circumvent this issue, we have utilized a metal-free, photomediated [3 + 2] cycloaddition reaction between diazo compounds and nitriles, leading to a step- and reagent-economical synthesis of bioactive oxazoles. These exciting developments guided us in the efficient synthesis of oxazole-based natural products like annuloline, alkaloids like pimprinethine, labradorin 2, and oxazole-containing pharmaceuticals such as oxaprozin.

Association of Serum Albumin and C-Reactive Protein with Outcomes in ESRD Patients Undergoing Hemodialysis at a Tertiary Care Center in Salem, Tamil Nadu.

Background: Chronic kidney disease (CKD) is a chronic condition characterized by irreversible renal dysfunction, leading to excretory and metabolic failure. Patients with end-stage renal disease (ESRD) often have elevated CRP and reduced albumin levels, causing inflammation. This study compares serum CRP, albumin, urea, creatinine, eGFR, blood pressure, and weight in patients on MHD with ESRD.

Anisotropy, topography and non-newtonian properties of cellular interiors probed by helical magnetic nanobots.

Unlabelled: Cells are building blocks of living systems. Spatio-temporal mapping of local biophysical changes within cells can lead to novel insights into various biological events. As demonstrated in previous works, successful internalization, controlled manipulation, bio-compatibility, and surface-functionalization capabilities make the helical magnetic nanobots, an ideal candidate for local intracellular measurements.

Exposure of Human Nasal Epithelial Cells to Polycyclic Aromatic Hydrocarbons Differentially Increases Markers of Inflammation and Expression of Aryl Hydrocarbon Pathway Genes.

Inhalation of polycyclic aromatic hydrocarbons (PAHs) derived from emissions of solid waste combustion is of public health concern, as PAHs are associated with respiratory cancers and inflammatory diseases. Here, we chose seven individual PAHs associated with gene expression changes induced by exposure to plastic incineration emission mixtures in human nasal epithelial cells in vitro and further elucidated inflammatory cytokine release and expression of aryl hydrocarbon (AhR) pathway gene response by these PAHs and ranked their toxicities. Overall ranking analyses showed differential inflammatory and gene expression potentials among PAHs, with high-molecular-weight PAHs inducing responses greater than those of their low-molecular-weight counterparts.

Diabetic foot attack: Managing severe sepsis in the diabetic patient.

Diabetic foot attack (DFA) is the most severe presentation of diabetic foot disease, with the patient commonly displaying severe sepsis, which can be limb or life threatening. DFA can be classified into two main categories: Typical and atypical. A typical DFA is secondary to a severe infection in the foot, often initiated by minor breaches in skin integrity that allow pathogens to enter and proliferate.

Effect of dietary fibre on the gastrointestinal microbiota during critical illness: A scoping review.

The systemic effects of gastrointestinal (GI) microbiota in health and during chronic diseases is increasingly recognised. Dietary strategies to modulate the GI microbiota during chronic diseases have demonstrated promise. While changes in dietary intake can rapidly change the GI microbiota, the impact of dietary changes during acute critical illness on the microbiota remain uncertain.

Elite genotype selection through antioxidant and antimicrobial activities, in vitro multiplication with -Topolin, genetic fidelity assessment via SCoT markers and cytological study, and chemo-profiling of selected elite genotype of Lam.: an underutilized alternative for nutrition and medicine.

Nowadays, the increasing world population and antibiotic resistance have become globally concerning issues. Lam. is an underexploited plant with multipurpose therapeutic and culinary uses and can be utilized as an alternative source of food and medicine.

Inhibition of Soluble Epoxide Hydrolase Confers Neuroprotection and Restores Microglial Homeostasis in a Tauopathy Mouse Model.

Background: The epoxyeicosatrienoic acids (EETs) are derivatives of the arachidonic acid metabolism with anti-inflammatory activities. However, their efficacy is limited due to the rapid hydrolysis by the soluble epoxide hydrolase (sEH). Accordingly, inhibition of sEH has been shown to stabilize the EETs and dampen neuroinflammation in Ab mouse models of Alzheimer's disease (AD).