Characterization of neural stem cells derived from human stem cells from the apical papilla undergoing three-dimensional neurosphere induction.

Objectives: The endogenous repairing based on the activation of neural stem cells (NSCs) is impaired by neurodegenerative diseases. The present study aims to characterize human stem cells from the apical papilla (hSCAPs) with features of mesenchymal stem cells (MSCs) and to demonstrate the neuronal differentiation of hSCAPs into NSCs through the formation of three-dimensional (3D) neurospheres, verifying the structural, immunophenotyping, self-renewal, gene expression and neuronal activities of these cells to help further improve NSCs transplantation.

Methodology: The hSCAPs were isolated from healthy impacted human third molar teeth and characterized as MSCs.