Molecular characterization and serodiagnostic evaluation of the Echinococcus ortleppi recombinant glutaredoxin 1 protein for cystic echinococcosis in buffalo (Bubalus bubalis).

Cystic echinococcosis (CE), caused by the metacestode of Echinococcus granulosus sensu lato (s.l.), adversely affects the physiology of the vital organs in which they grow.

Analysis of the epidemiological variables associated with a high prevalence of bovine surra in the Chhattisgarh state of India.

Trypanosoma evansi, a unicellular haemoflagellate, causes surra in bovines and other economically important livestock species. We report here the epidemiological variables associated with the high prevalence of T. evansi infection in cattle in the plain agro-climatic zone of Chhattisgarh state, India.

Evaluation of the Immunoprotective Potential of Recombinant Paraflagellar Rod Proteins of in Mice.

Trypanosomosis, caused by , is an economically significant disease of livestock. Systematic antigenic variation by the parasite has undermined prospects for the development of a protective vaccine that targets the immunodominant surface antigens, encouraging exploration of alternatives. The paraflagellar rod (PFR), constituent proteins of the flagellum, are prominent non-variable vaccine candidates for owing to their strategic location.

High seroprevalence of Toxoplasma gondii in goats in Jharkhand state of India.

Toxoplasmosis, caused by Toxoplasma gondii, is an important food borne zoonosis worldwide. Although goat meat constitutes an important dietary protein source, improperly cooked meat is a potential source of infection to humans. Data on prevalence of toxoplasma in goat is scanty from India.

Molecular Cloning, Expression and Characterization of Para Flagellar Rod Protein 1 of .

Background: Antigenic variation allows the trypanosomes to evade the potentially destructive host immune response and is an important reason for failure to develop a protective vaccine. Among the non-variant structural proteins, paraflagellar rod protein (PFR) is a prospective vaccine target owing to its role in the active movement of the parasite.

Methods: The PFR1 gene was cloned in pET-32a expression vector and after confirmation by restriction digestion, expressed as a Histidine-tagged fusion protein, in BL21 DE3 strain of .

Important hemoprotozoan diseases of livestock: Challenges in current diagnostics and therapeutics: An update.

Hemoprotozoan parasites pose a serious threat to the livestock population in terms of mortality, reduced milk yield and lowered draft power. Diagnosis of these diseases often poses a challenging task. Needless to say that impact of disease in health and productivity is huge though a fair economic assessment on the quantum of economic loss associated is yet to be worked out from India.

Protective antibody and cytokine responses in mice following immunization with recombinant beta-tubulin and subsequent Trypanosoma evansi challenge.

Background: Trypanosomosis or Surra, caused by the flagellated hemoprotozoan parasite Trypanosoma evansi, is a disease of economic importance through its wide prevalence in domestic livestock in tropical countries. In the absence of a protective vaccine, management of the disease relies on a few available chemotherapeutic agents. Although humoral immunity is the mainstay of resistance to T.

MOLECULAR CHARACTERIZATION AND SEQUENCE PHYLOGENETIC ANALYSIS OF SURFACE ANTIGEN 3 (SAG3) GENE OF LOCAL INDIAN ISOLATES (CHENNAI AND IZATNAGAR) OF Toxoplasma gondii.

Context And Objective: The molecular characterization of local isolates of Toxoplasma gondii is considered significant so as to assess the homologous variations between the different loci of various strains of parasites.

Design And Setting: The present communication deals with the molecular cloning and sequence analysis of the 1158 bp entire open reading frame (ORF) of surface antigen 3 (SAG3) of two Indian T. gondii isolates (Chennai and Izatnagar) being maintained as cryostock at the IVRI.

Serodiagnosis of Toxoplasma gondii infection in bovines from Kerala, India using a recombinant surface antigen 1 ELISA.

Data on the prevalence of toxoplasmosis in farm animals from India is scanty. Though a few reports exist on prevalence of toxoplasmosis in small ruminants, information on toxoplasmosis in large ruminants is virtually nonexistent from India. An antibody detection recombinant ELISA specific for Toxoplasma gondii was laboratory standardized using recombinant surface antigen 1 (SAG1) protein.

Detection of antibodies to Toxoplasma gondii in domesticated ruminants by recombinant truncated SAG2 enzyme-linked immunosorbent assay.

An antibody detection recombinant enzyme-linked immunosorbent assay (ELISA) specific for Toxoplasma gondii was laboratory standardized using recombinant truncated surface antigen 2 (SAG2) protein of T. gondii. A 483-bp sequence coding for truncated tachyzoite stage-specific SAG2 protein was amplified and ligated in pPROExHT-b expression vector to transform Escherichia coli DH5α cells.

Molecular characterization of surface antigen 3 (SAG3) gene of Toxoplasma gondii RH-IVRI strain.

Toxoplasmosis, caused by Toxoplasma gondii, is a zoonotic disease and is responsible for abortions, stillbirth, and neonatal complications in livestock, especially in sheep, goats, and pigs. The molecular characterization of the parasite having global distribution is considered important to delineate the phylogenetic relationship among different isolates/strains of the parasite. The present communication deals with the molecular cloning and sequence analysis of the 1,158 bp entire open reading frame of surface antigen 3 (SAG3) of T.

Sero-surveillance for surra in cattle using native surface glycoprotein antigen from Trypanosoma evansi.

Surra, caused by Trypanosoma evansi affects a wide range of domestic and wild animals in the tropics, taking a huge toll on the already impoverished economy here. In bovines surra normally develops into a chronic infection that is often associated with severe production losses, yet with no distinct clinical signs making its adequate diagnosis vital. Though direct microscopic observation of T.

Induction of protective immune response in mice by a DNA vaccine encoding Trypanosoma evansi beta tubulin gene.

Surra, caused by Trypanosoma evansi, is an economically important veterinary disease of the tropics. Lack of effective drugs or vaccines have made surra a severe economic burden particularly in Asia and sub-Saharan Africa. In this study, a naked DNA construct encoding full length T.