Publications by authors named "Anujin Batbold"
Commun Biol
September 2024
Article Synopsis
- The study presents a new phagemid-based system that produces CRISPR-Cas13a-loaded antibacterial capsids (AB-capsids) specifically targeting multidrug-resistant Staphylococcus aureus.
- By optimizing phagemid copy numbers, researchers achieved higher yields and purity of AB-capsids, illustrating a direct relationship between phagemid quantity and capsid production.
- The developed AB-capsids effectively eliminate targeted S. aureus strains while leaving non-target strains unharmed, showcasing their potential as effective tools against antibiotic-resistant bacteria.
Article Synopsis
- The lab created a phagemid system to generate CRISPR-Cas13a-antimicrobial capsids specifically targeting MRSA to combat rising antimicrobial resistance.
- They faced a challenge with unwanted wild-type phage production during packaging, which was addressed by introducing silent mutations to reduce contamination while maintaining efficiency.
- The optimized system showed effective sequence-specific killing of MRSA strains but highlights the need for further research on its effectiveness against other bacteria and in real body conditions.
Article Synopsis
- The study focuses on oxacillin-susceptible methicillin-resistant Staphylococcus aureus (OS-MRSA), which poses new treatment challenges due to its ability to develop resistance through chromosomal mutations.
- Researchers analyzed six mutant strains with decreased oxacillin susceptibility, examining how mutations in RNA polymerase (RNAP) genes led to transcription dysfunction and an accumulation of certain metabolites.
- The findings indicated that these mutations resulted in cell wall thickening, which ultimately decreased the mutants' susceptibility to β-lactam antibiotics, highlighting the need for a better understanding of these mechanisms for effective clinical management.
