Localization, induction, and cellular effects of tau phosphorylated at threonine 217.

Introduction: Tau phosphorylation at T217 is a promising Alzheimer's disease (AD) biomarker, but its functional consequences were unknown.

Methods: Human brain and cultured mouse neurons were analyzed by immunoblotting and immunofluorescence for total tau, tau , tau , tau , and tau . Direct stochastic optical reconstruction microscopy (dSTORM) super resolution microscopy was used to localize tau in cultured neurons.

Panama: An Open-Source Educational App for Ion Channel Biophysics Simulation.

This article describes an open-source educational software, called Panama, developed using R, that simulates the biophysics of voltage-gated ion channels. It is made publicly available as an R package called Panama and as a web app at http://www.neuronsimulator.