Surgical management of complicated duplex kidney: A tertiary referral centre 10-year experience.

Aim: The management of a complicated duplex kidney remains a challenge for paediatric urologists. The aim of this study is to report a 10-year experience of the surgical management of complicated duplex kidney in a single tertiary care paediatric referring hospital.

Materials And Methods: Clinical records of all children who undergone a surgical procedure for complicated duplex systems between January 2009 and March 2019 at our institution were retrospectively reviewed.

Inhibition of SRC-mediated integrin signaling in bone marrow niche enhances hematopoietic stem cell function.

Interaction with microenvironmental factors is crucial for the regulation of hematopoietic stem cell (HSC) function. Stroma derived factor (SDF)-1α supports HSCs in the quiescent state and is central to the homing of transplanted HSCs. Here, we show that integrin signaling regulates expression transcriptionally.

Comparison of CD34 cell mobilization, blood graft cellular composition, and post-transplant outcome in myeloma patients mobilized with filgrastim or pegfilgrastim added to low-dose cyclophosphamide: A prospective multicenter study.

Background: Scarce data exist on the impact of granulocyte-colony stimulating factor (G-CSF) type on the mobilizing capacity of CD34 cells, graft cellular composition, and outcome in myeloma (MM) patients.

Patients And Methods: In this prospective multicenter study, 70 patients with MM received filgrastim (FIL) and 20 patients received pegfilgrastim (PEG) as a G-CSF after low-dose cyclophosphamide. Flow cytometry was used to analyze the mobilization of CD34 cells and cellular composition of blood grafts, hematologic recovery, and survival after auto-SCT according to the G-CSF choice.

A multibiomarker approach to assess lead toxicity on the black clam, Villorita cyprinoides (Gray, 1825), from Cochin estuarine system (CES), southwest coast, India.

The southwest coast of India along the Cochin region is seriously affected by metal contamination from increasing industrial activities. This contribution pertains to the chronic toxicity effects of lead and its biomarker responses on the black clam, Villorita cyprinoides, largely endemic to Southern India. The metal concentration (lead (Pb), copper (Cu), zinc (Zn), cadmium (Cd), nickel (Ni), and chromium (Cr)) and contamination indices such as geoaccumulation index and contamination factor suggests that the Cochin estuarine system (CES) is moderately to heavily polluted with metals.

Expression of Leptospira membrane proteins Signal Peptidase (SP) and Leptospira Endostatin like A (Len A) in BL-21(DE3) is toxic to the host cells.

Heterologous expression of Integral Membrane Proteins (IMPs) is reported to be toxic to the host system in many studies. Even though there are reports on various concerns like transformation efficiency, growth properties, protein toxicity, inefficient expression and protein degradation in IMP overexpression, no studies so far addressed these issues in a comprehensive way. In the present study, two transmembrane proteins of the pathogen , namely Signal peptidase (SP), and Leptospira Endostatin like A (Len-A) were taken along with a cytosolic protein Hydrolase (HYD) to assess the differences in transformation efficiency, protein toxicity, and protein stability when over expressed in ().

Toxicity effects of zinc on two marine diatoms, under varying macronutrient environment.

Diatoms constitute one of the fundaments in the trophic food chains and are responsible for much of the bioaccumulation of heavy metals, in particular zinc, throughout the web of food chains. Chaetoceros calcitrans and Thalassiosira weissflogii are widely present as a dominant marine diatom in estuarine and coastal waters. The zinc uptake, its fractionation in subcellular structures and macromolecular compartments in two marine diatoms, C.

Heterologous Expression, Purification and Characterization of an Oligopeptidase A from the Pathogen Leptospira interrogans.

Oligopeptidases are enzymes involved in the degradation of short peptides (generally less than 30 amino acids in size) which help pathogens evade the host defence mechanisms. Leptospira is a zoonotic pathogen and causes leptospirosis in mammals. Proteome analysis of Leptospira revealed the presence of oligopeptidase A (OpdA) among other membrane proteins.

Elderly and depression.

It is believed that it is 'normal' for the elderly per sons to become depressed and many cases aren't recognized and thus lead to untold misery for the sufferers and their families. We have found that majority of the clients under study were females belonging to low socio economic status, uneducated and unemployed. Majority of the clients had moderate depression (58%), And about 19% of them had severe depression.