Publications by authors named "Anu Kalia"

For the extraction of sugars and their subsequent conversion into ethanol, removing lignin from sugarcane bagasse is a major challenge attributed to its recalcitrant nature. This study compares the efficacy of green deep eutectic solvents with conventional acid/alkali pre-treatments for the delignification of sugarcane bagasse. Among different deep eutectic solvent pre-treatments, the maximum removal of lignin i.

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The rise of direct-seeded rice cultivation as a suitable alternative to transplanted puddled rice depends on developing genotypes with high seedling emergence under deep sown conditions. Two rice genotypes (IRGC 128442 and PR126) were screened for contrasting seedling emergence and subjected to high-throughput RNA sequencing under varying sowing depths (4 cm and 10 cm) and time intervals (5, 10, and 15 days after sowing). On average, a total of 2702 differentially expressed genes were identified across twelve inter- and intra-genotypic pairwise differential expression analyses, with a false discovery rate ≤ 0.

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Article Synopsis
  • * Researchers developed a genetically modified sugarcane resistant to red rot by introducing an endochitinase gene from Trichoderma spp., resulting in a six-fold increase in gene expression compared to normal plants.
  • * The resistant transgenic plant (Chit 3-13) demonstrated improved sugar retention and disease resistance, indicating its potential for breeding programs to combat red rot and minimize losses in sugar content.
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Macrofungi commonly referred to as Mushrooms are distributed worldwide and well known for their nutritional, medicinal, and organoleptic properties. Strain improvement in mushrooms is lagging due to paucity of efficient genome modification techniques. Thus, for advanced developments in research and commercial or economical viability and benefit, CRISPR/Cas9 (clustered regularly interspaced short palindromic repeats/CRISPR-associated nuclease 9) emerged as an efficient genome editing tool.

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Nano-scale particles (NPs) have gained increased interest as non-viral vectors for nucleic acid delivery due to their ability to penetrate through unabraded cell membranes. The previous studies performed have evaluated the nanomaterials for their microbial transformation proficiency but have not compared the relative efficacy. The present study aims to identify the most proficient nano-delivery vehicle among the chemically synthesized/functionalized non-metal oxide, metal/metal oxide, and carbon-based (carbon nanotube (CNT), graphene oxide (GO)) nanomaterial(s) (NMs) for the transformation of two gram-negative bacteria, i.

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India's livestock industry is grappling with a shortage of green fodder, necessitating concerted efforts to boost organized production and ensure a sufficient supply of high-quality forages, crucial for formulating nutritionally balanced, cost-effective, and rumen-healthy animal diets. Hence, this study was conducted to assess the plant growth-promoting characteristics of liquid microbial inoculants and their impact on the yield of forage pearl millet. The bacterial cultures utilized included Sphingobacterium sp.

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The current study was aimed for the generation of Pleurotus extracellular extract-mediated selenium and zinc-oxide nanoparticles (NPs). The Pleurotus djamor (PD) and Pleurotus sajor-caju (PSC) extracts were incubated with different concentrations of sodium selenate and zinc acetate to yield BioSeNPs and BioZnONPs. The NPs formation led to visual color change (brick-red and white for Se and Zn nanosols, respectively).

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Potato (Solanum tuberosum L.), an important horticultural crop is a member of the family Solanaceae and is mainly grown for consumption at global level. Starch, the principal component of tubers, is one of the significant elements for food and non-food-based applications.

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Essential oils are highly complex volatile chemical compounds utilized for food preservation. The present study compares the antibacterial, and antibiofilm activities of essential oils (EOs) and their blends. Three EOs-basil, clove, and lemongrass-and their blends were evaluated against five food-borne bacterial pathogens.

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In the context of degradation of soil health, environmental pollution, and yield stagnation in the rice-wheat system in the Indo-Gangetic Plains of South Asia, an experiment was established in split plot design to assess the long-term effect of crop residue management on productivity and phosphorus requirement of wheat in rice-wheat system. The experiment comprised of six crop residue management practices as the main treatment factor with three levels (0, 30 and 60 kg PO ha) of phosphorus fertilizer as sub-treatments. Significant improvement in soil aggregation, bulk density, and infiltration rate was observed under residue management (retention/incorporation) treatments compared to residue removal or residue burning.

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Titanium dioxide nanoparticles (TiO NPs) are among the most commonly used nanomaterials and are most likely to end up in soil. Therefore, it is pertinent to study the interaction of TiO NPs with soil microorganisms. The present in vitro broth study evaluates the impacts of low-dose treatments (0, 1.

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Background: Pleurotus has a remarkable nutritional and nutraceutical profile due to mineral mobilization and accumulation abilities from the substrate. The present study aimed to observe the effect of single and dual supplementations Se and Zn on biochemical parameters of P. florida, P.

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Unlabelled: The mycosynthesis of biogenic NPs using nanotechnology technique is an ecofriendly and economical approach. The extracellular mycelial extract of the fungi were used to biosynthesized Zn, Cu and Fe NPs using zinc sulphate, zinc chloride, copper sulphate, copper chloride ferrous sulphate and ferric chloride, precursor salts at 1.0 mM concentration.

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Purpose: This manuscript aimed for the generation of γ-irradiation derived mutants of potato genotype PAU/RR-1501 possessing desirable processing traits.

Materials And Methods: Nodal cuttings from virus-free explants were established on basal MS medium and irradiated with different doses (0, 5, 10 and 20 Gy) of γ-irradiation. The 5 and 10 Gy treated plantlets were multiplied and used for micro-tuber induction.

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Late blight (LB) is a serious disease that affects potato crop and is caused by Phytophthora infestans. Fungicides are commonly used to manage this disease, but this practice has led to the development of resistant strains and it also poses serious environmental and health risks. Therefore, breeding for resistance development can be the most effective strategies to control late blight.

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Sporadic burning of rice straw and the particulate air pollution caused consequently have created a pressing need for identification of practical environmentally sound rice residue management methods. However, the agronomic interventions associated with the agri-inputs particularly the type of nitrogen fertilizer source must be worked out for these interventions. In a two-year field study performed at two different locations representing sandy loam and clay loam soil types, zero tillage with application of nitrophosphate (applied as basal dose through drilling) in combination with urea (applied at 1st irrigation + 3 foliar sprays of urea at weekly interval) significantly enhanced the grain and straw yield of wheat.

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The spotted bollworm (Lepidoptera: Nolidae) is a polyphagous pest with enormous economic significance, primarily affecting cotton and okra. However, the lack of gene sequence information on this pest has a significant constraint on molecular investigations and the formulation of superior pest management strategies. An RNA-seq-based transcriptome study was conducted to alleviate such limitations, and de novo assembly was performed to obtain transcript sequences of this pest.

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Devising and consolidating cost-effective and greener technologies for sustainable energy production pertain to some of the most pressing needs of the present times. Bioconversion of abundantly available lignocellulosic materials into fermentable sugars to produce biofuels involves the cost-extensive requirement of hydrolytic enzymes called cellulases. Cellulases are highly selective and eco-friendly biocatalysts responsible for deconstruction of complex polysaccharides into simple sugars.

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Biochar (BC) is a carbon-rich material that can be obtained by thermal decomposition of agricultural solid waste under oxygen-limited conditions. It has received increasing attention as a cost-effective sorbent to treat metal-contaminated water due to attributes such as high porosity and the presence of various functional groups. The heavy metal (HM) sorption and removal capacity of BC can be enhanced by developing novel biochar nanohybrids (BNHs) that can be produced via surface modification of BC with nanomaterials.

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Rice is an important food crop for three billion people worldwide. The crop is vulnerable to several diseases. Sheath blight caused by fungal pathogen Rhizoctonia solani is a significant threat to rice cultivation accounting for up to 50% yield losses.

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The efficient removal of lignin and silica from paddy straw is essential for its volarization into biofuels and other value-added products. In this work, different chemical pre-treatments viz. acid/alkali, organosolv and deep eutectic solvents were carried out to assess the extent of delignification and desilication of paddy straw.

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Traditional nanoparticle (NP) synthesis methods are expensive and generate hazardous products. It is essential to limit the risk of toxicity in the environment from the chemicals as high temperature and pressure is employed in chemical and physical procedures. One of the green strategies used for sustainable manufacturing is microbial nanoparticle synthesis, which connects microbiology with nanotechnology.

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Titanium dioxide (TiO) nanoparticles (NPs) are the most widely used nanomaterials and their expanding use raises concerns about their impacts on soil ecosystems and functioning. The present study evaluates the potential impacts of TiO NPs applied at low doses (0, 1.0, 2.

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Mycogenic silver nanoparticles (AgNPs) produced by some biocontrol agents have shown the ability to inhibit the growth of numerous plant pathogenic fungi, which may be a unique method of disease management. This study describes the extracellular production of AgNPs by . The size, shape, charge, and composition of the AgNPs were subsequently studied by UV-visible spectroscopy, DLS, zeta potential, TEM, SEM, and EDX, among other methods.

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