AI Techniques to Identify Nerve Cell Alterations in Digital Images.

Artificial intelligence (AI), utilising computing power, has managed to influence the health sector with many applications based on algorithms, tools, and automated processes. In this work, neuronbiological images acquired by an electronic microscope are assessed under appropriate image processing to identify areas of interest. The algorithmic steps identified the alterations of nerve cells that appeared as areas in the red channel of each digital image.

Molecular Mechanisms Employed by Neurons to Receive and Transduce Signals Essential for Learning and Memory.

The ability to learn from the consequences of one's actions, extracting useful information from threatening, painful or rewarding encounters and developing the skill of predicting probable events from pre-experienced stimuli, is essential for survival and reproductive success [...

Tag1 deficiency results in olfactory dysfunction through impaired migration of mitral cells.

The olfactory system provides mammals with the abilities to investigate, communicate and interact with their environment. These functions are achieved through a finely organized circuit starting from the nasal cavity, passing through the olfactory bulb and ending in various cortical areas. We show that the absence of transient axonal glycoprotein-1 (Tag1)/contactin-2 (Cntn2) in mice results in a significant and selective defect in the number of the main projection neurons in the olfactory bulb, namely the mitral cells.

The expression of TAG-1 in glial cells is sufficient for the formation of the juxtaparanodal complex and the phenotypic rescue of tag-1 homozygous mutants in the CNS.

Myelinated fibers are organized into specialized domains that ensure the rapid propagation of action potentials and are characterized by protein complexes underlying axoglial interactions. TAG-1 (Transient Axonal Glycoprotein-1), a cell adhesion molecule of the Ig superfamily, is expressed by neurons as well as by myelinating glia. It is essential for the molecular organization of myelinated fibers as it maintains the integrity of the juxtaparanodal region through its interactions with Caspr2 and the voltage-gated potassium channels (VGKCs) on the axolemma.

Impairment of learning and memory in TAG-1 deficient mice associated with shorter CNS internodes and disrupted juxtaparanodes.

The cell adhesion molecule TAG-1 is expressed by neurons and glial cells and plays a role in axon outgrowth, migration and fasciculation during development. TAG-1 is also required for the clustering of Kv1.1/1.

Neonatal handling and gender modulate brain monoamines and plasma corticosterone levels following repeated stressors in adulthood.

Neonatal handling affects the response to repeated stress in a sexually dimorphic manner. In order to elucidate the mechanisms underlying these gender-dependent effects, we investigated the consequences of neonatal androgenization and handling on adult stress reactivity by determining: (a) immobility time during repeated forced swimming, (b) plasma corticosterone levels, and (c) brain serotonin and dopamine levels and turnover after either repeated forced swimming, or repeated forced swimming followed by repeated restraint stress. In neonatally androgenized females, immobility time was lower in the handled than in the non-handled rats, a pattern resembling that of the males, suggesting that the sexually dimorphic effect of handling on immobility time can be attributed to the organizational effects of testosterone.

Late granule cell genesis in quail cerebellum.

Proliferation of avian cerebellar neurons, including granule cells, is thought to be completed during embryonic life, and aspects of cell addition in cerebellar lobules in posthatching life are unknown. The present study tested the hypothesis that cell genesis in late embryonic and posthatching stages of quail cerebellum occurs in parallel with the performance of motor programs. After exposure to bromodeoxyuridine, short (20 hours) and long survival time points were selected to investigate survival and migration of labeled cells.