Micropropagation and in vitro rejuvenation of F. Muell.

Micropropagation is an important tool for the propagation for recalcitrant species, like This is a first reported about a complete study of the , it includes the vegetative rescue protocol of mature trees, its micropropagation up to the implementation of a mini-garden and clonal micro-garden and evaluation of adventitious rooting of their propagules. The in vitro rejuvenation of genotypes (03, 05, 06, 13 and 15), over 21 successive subcultures, was evaluated by comparing minicutting and microcutting techniques and the efficiency of this biotechnological tool for cloning of the mature trees. The results provide evidence of the in vitro rejuvenation occurring in the mature trees during micropropagation, leading to increases in the multiplication ratios of the microstumps of three genotypes (03, 05 and 15).

Analysis of nutrient deficiencies affecting in vitro growth and development of Maiden.

Although basal medium optimization is a key factor in the success of tissue culture, its mineral composition is frequently disregarded when optimizing in vitro propagation protocols. A previous work on micropropagation suggests that excessive callus formation and leaf chlorosis are related to specific nutritional conditions of the basal media. Recently, a novel basal medium based on the mineral nutrient analysis of young stump shoots was developed and successfully tested in plant regeneration and micropropagation of , avoiding all these issues.