Publications by authors named "Antonio Cordova"
The modular nature of polyketide assembly lines and the significance of their products make them prime targets for combinatorial engineering. The recently updated module boundary has been successful for engineering short synthases, yet larger synthases constructed using the updated boundary have not been investigated. Here we describe our design and implementation of a BioBricks-like platform to rapidly construct 5 triketide, 25 tetraketide, and 125 pentaketide synthases to test every module combination of the pikromycin synthase.
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- The study focuses on an extremophile that maintains a highly organized nucleoid, which may help it tolerate ionizing radiation (IR).
- A new high-throughput method combining confocal microscopy and computational modeling is introduced to quantitatively analyze nucleoid and cell geometry under IR stress.
- Results show that IR exposure leads to nucleoid compaction, alters cell morphology, and suggests potential roles for nucleoid-associated proteins in regulating these changes.
The modular nature of polyketide assembly lines and the significance of their products make them prime targets for combinatorial engineering. While short synthases constructed using the recently updated module boundary have been shown to outperform those using the traditional boundary, larger synthases constructed using the updated boundary have not been investigated. Here we describe our design and implementation of a BioBricks-like platform to rapidly construct 5 triketide, 25 tetraketide, and 125 pentaketide synthases from the updated modules of the Pikromycin synthase.
View Article and Find Full Text PDF