Publications by authors named "Antonio Alfonso De Lauri"
Methods Protoc
September 2023
Article Synopsis
- The study presents an optimized electroporation protocol for transforming yeast cells with whole-genome libraries, significantly increasing transformation efficiency to about 10 cfu/µg plasmid DNA.
- The method enables the use of small quantities of genomic DNA (0.1 µg per reaction), achieving 10-100× coverage of diverse libraries and offers detailed calculations for estimating library size and transformation efficiency.
- Validation through nanopore sequencing and expression analysis confirms the protocol maintains library size, diversity, and functionality, making it suitable for efficient downstream screening with minimal genomic library input.
