Publications by authors named "Antonia Klemt"
Article Synopsis
- The text discusses how cellular membrane balance (homoeostasis) is maintained through regulated transport processes between organelles involved in endocytosis and secretion.
- This process is facilitated by adaptor protein complexes (APs) that are recruited by the small GTPase ARF1, aiding in cargo selection for membrane trafficking.
- The study identifies two distinct roles of ARF1 compartments in facilitating secretory cargo export from the Golgi and recycling of materials from endosomes, challenging the conventional understanding of vesicle transport.
In recent years, proximity labeling has established itself as an unbiased and powerful approach to map the interactome of specific proteins. Although physiological expression of labeling enzymes is beneficial for the mapping of interactors, generation of the desired cell lines remains time-consuming and challenging. Using our established pipeline for rapid generation of C- and N-terminal CRISPR-Cas9 knock-ins (KIs) based on antibiotic selection, we were able to compare the performance of commonly used labeling enzymes when endogenously expressed.
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