Beta(2)-adrenergic receptor gene delivery to the endothelium corrects impaired adrenergic vasorelaxation in hypertension.

Background: Impaired beta-adrenergic receptor (AR)-mediated vasorelaxation in hypertension plays a role in increased peripheral vascular resistance and blood pressure. Because the beta(2)AR is the most abundant vascular AR subtype, we sought to enhance betaAR vasorelaxation by overexpressing beta(2)ARs via adenoviral-mediated gene transfer (ADbeta(2)AR) to the vascular endothelium of the carotid artery.

Methods And Results: In normotensive Wistar-Kyoto (WKY) and spontaneously hypertensive (SHR) rats, we exposed the right common carotid artery to ADbeta(2)AR in situ for 15 minutes by injection into the lumen while the blood flow was interrupted.