Publications by authors named "Antonia Babl"
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) in Macrophages.
Methods Mol Biol
August 2024
Cleavage Under Targets and Release Using Nuclease (CUT&RUN) is a method to detect specific interactions between DNA and DNA-associated proteins. It is valuable for the characterization of the binding of transcription factors or co-regulators genome wide. Furthermore, it can be used for epigenetic profiling, chromatin accessibility assessment, and identification of regulatory elements.
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- High-resolution NMR studies of membrane proteins are difficult due to size-related signal issues, but segmental isotope labeling can help simplify the process.
- A stabilized split-intein system allows for effective protein trans-splicing under challenging conditions, enhancing the ability to label integral membrane proteins.
- This method improves NMR analysis by producing high-quality spectra in detergent micelles and lipid nanodiscs, making it valuable for studying the structures and dynamics of various proteins.