Effect of Group Mixing and Available Space on Performance, Feeding Behavior, and Fecal Microbiota Composition during the Growth Period of Pigs.

Stress significantly affects the health, welfare, and productivity of farm animals. We performed a longitudinal study to evaluate stress's effects on pig performance, feeding behavior, and fecal microbiota composition. This study involved 64 Duroc pigs during the fattening period, divided into two experimental groups: a stress group ( = 32) and a control group ( = 32).

Effect on Feeding Behaviour and Growing of Being a Dominant or Subordinate Growing Pig and Its Relationship with the Faecal Microbiota.

Pigs are a social species, and they establish hierarchies for better use of resources and to reduce conflicts. However, in pig production, the opportunities for growth can differ between dominant and subordinate animals. In the present study, a system was tested to perform a dominant versus subordinate test in growing pigs to investigate how the hierarchy affects feeding behaviour, growth, and gut microbiota assessed in faeces.

Correction: Mixed management in growing and finishing pigs: Differences between gender and their impacts on behavior, growth performance, and physiological parameters.

[This corrects the article DOI: 10.1371/journal.pone.

Reducing Stocking Densities and Using Cooling Systems for More Adapted Pigs to High Temperatures When Reared in Intensive Conditions.

This study is aimed at evaluating the effect of reducing stocking density and using cooling systems to mitigate the negative effects of high temperatures in growing pigs (females and castrated males) reared in intensive conditions (from 25 to 100 kg) during summer (June to October 2020). The experimental design was a 2 × 2 factorial where pigs were provided with an evaporative cooling system and/or raised at regular or at lower stocking densities (i.e.

Copy Number Variation on Loci Affects the Diversity and Composition of the Fecal Microbiota in Pigs.

Genetic variation in the pig genome partially modulates the composition of porcine gut microbial communities. Previous studies have been focused on the association between single nucleotide polymorphisms (SNPs) and the gut microbiota, but little is known about the relationship between structural variants and fecal microbial traits. The main goal of this study was to explore the association between porcine genome copy number variants (CNVs) and the diversity and composition of pig fecal microbiota.

Mixed management in growing and finishing pigs: Differences between gender and their impacts on behavior, growth performance, and physiological parameters.

Mixing, a common management strategy used to regroup pigs, has been reported to impair individual performance and affect pig welfare because of the establishment of a new social hierarchy after regrouping. In this study we aimed to determine whether mixing management (non-mixed vs. mixed) and gender (gilts vs.

Hierarchy Establishment in Growing Finishing Pigs: Impacts on Behavior, Growth Performance, and Physiological Parameters.

In recent years, an increased number of studies have dealt with the analysis of social dominance related to animal behavior, physiology, and performance. This study aimed to investigate whether hierarchical ranking affects the coping style, non-social behavior during open field and novel object tests, performance, and physiological parameters of pigs. A total of 48 growing pigs (24 barrows and 24 females) were mixed three times during the growing-finishing period.

Induced Stress and Tactile Stimulation Applied to Primiparous does and their Consequences on Maternal Behavior, Human-Animal Relationships, and Future Offspring's Sexual Disorders.

The aim of this study was to investigate whether tactile stimulation in rabbits during the gestation phase improve the maternal behavior and human-animal relationships as well as the effects on reproductive behavior of male kits when reached maturity compared to induced stress. A total of 33 primiparous New Zealand does were selected after pregnancy confirmation and allocated in a randomized complete block design. The treatments applied were as follows: (C) animals not stimulated during the experimental period; (TS) animals that received tactile stimulation; and (SS) does which were immobilized.

Consumers' Expectations about Meat from Surgical Castrated or Immunocastrated Male and Female Iberian Pigs.

A common practice in Iberian pigs is the castration of both males and females, and it can be carried out surgically or by immunization against gonadotropin-releasing factor (GnRF). The aim of this work was to determine consumers' overall liking and expectations towards Iberian pork from five different sex types (castrated females, entire females, GnRF-vaccinated females, castrated males and GnRF-vaccinated males), as well as to know the attitudes and beliefs of consumers towards castration and immunocastration. Loins from 83 Iberian pigs were collected and evaluated by 252 consumers in Barcelona and Madrid.

Leveraging host-genetics and gut microbiota to determine immunocompetence in pigs.

Background: The gut microbiota influences host performance playing a relevant role in homeostasis and function of the immune system. The aim of the present work was to identify microbial signatures linked to immunity traits and to characterize the contribution of host-genome and gut microbiota to the immunocompetence in healthy pigs.

Results: To achieve this goal, we undertook a combination of network, mixed model and microbial-wide association studies (MWAS) for 21 immunity traits and the relative abundance of gut bacterial communities in 389 pigs genotyped for 70K SNPs.

Intramuscular vs. Intradermic Needle-Free Vaccination in Piglets: Relevance for Animal Welfare Based on an Aversion Learning Test and Vocalizations.

The aim of the present study was to compare intramuscular injection with a needle and intradermic needle-free vaccinations against porcine reproductive and respiratory syndrome (PRRS) in piglets at 28 days old by studying behavioral and physiological reactions. A total of 72 piglets divided into 2 sex-balanced batches were assessed. Within each batch, the piglets were divided into three treatments, which were Hipradermic (0.

Should Animal Welfare be Included in Educational Programs? Attitudes of Secondary and University Students from Eight EU Countries.

Animal Welfare (AW) educational programs aim to promote positive attitudes of future generations toward animal production systems. This study investigated whether secondary and university students in the majors that are not related to AW teaching believe that this concept should be included also in their educational programs. The determinant factors affecting students' attitudes toward such a decision were analyzed.

A gene co-association network regulating gut microbial communities in a Duroc pig population.

Background: Analyses of gut microbiome composition in livestock species have shown its potential to contribute to the regulation of complex phenotypes. However, little is known about the host genetic control over the gut microbial communities. In pigs, previous studies are based on classical "single-gene-single-trait" approaches and have evaluated the role of host genome controlling gut prokaryote and eukaryote communities separately.

Gut eukaryotic communities in pigs: diversity, composition and host genetics contribution.

Background: The pig gut microbiome harbors thousands of species of archaea, bacteria, viruses and eukaryotes such as protists and fungi. However, since the majority of published studies have been focused on prokaryotes, little is known about the diversity, host-genetic control, and contributions to host performance of the gut eukaryotic counterparts. Here we report the first study that aims at characterizing the diversity and composition of gut commensal eukaryotes in pigs, exploring their putative control by host genetics, and analyzing their association with piglets body weight.

Sex Differences in Group Composition and Habitat Use of Iberian Free-Range Pigs.

The aim of the present work was to study group size, group composition and habitat use of Iberian pigs along the year when reared outdoor. This consists of a regimen in which animals are reared free range from 2 months of age until at least 14 months of age. In a first stage, animals are supplemented with concentrates, and in a second, called montanera, pigs eat just natural resources in areas with no more than two pigs per hectare.

To Provide a Double Feeder in Growing Pigs Housed under High Environmental Temperatures Reduces Social Interactions but Does Not Improve Weight Gains.

Heat stress and competition for food are two major challenges in pigs reared in intensive conditions. The aim of the present work was to study the effect of providing a double feeder for pigs reared under two different environmental temperatures. In addition, two types of flooring, of 100% slat and 30% slat 70% concrete, were also considered.

Animal Welfare Assessment Protocol for Does, Bucks, and Kit Rabbits Reared for Production.

Rabbits are the third species in terms of number of animals reared for meat production in the world. However, in comparison to other species, very few studies have focused on their welfare. The aim of the present study is to implement an animal welfare-assessment protocol developed through a multidimensional approach and containing a number of animal-based measures for bucks, does, and kit rabbits.

Testing an Animal Welfare Assessment Protocol for Growing-Rabbits Reared for Meat Production Based on the Welfare Quality Approach.

The objective of the present study is to present an animal welfare assessment protocol for growing-rabbits for discussion after its implementation in 32 farms from Spain and Portugal. The protocol comprises the principles of Good Feeding, Good Housing, Good Health and Appropriate Behaviour of the Welfare Quality protocols and includes 36 welfare parameters. Overall, the protocol showed a good capacity for discrimination between farms, with scores ranging 44 to 82 points.

Environmental and Management Factors Affecting the Time Budgets of Free-Ranging Iberian Pigs Reared in Spain.

Understanding the natural behaviour of pigs in free-range conditions facilitates interpretation of their behaviour in intensive conditions. Studying six different farms over two years at different seasons, with climatic and management variations, allowed for a general description of Iberian pig behaviour and which factors have an influence on it. The main activity found was resting (56.

Should Animal Welfare Regulations Be More Restrictive? A Case Study in Eight European Union Countries.

Increasingly, intensive livestock production systems have increased societal concern regarding the current animal welfare standards. We investigated whether individuals in their roles as consumers and citizens believe that the current European regulations regarding animal welfare should be more restrictive. Factors affecting this decision were assessed by analyzing respondents' understanding of animal welfare-related issues, their subjective and objective knowledge levels, the credibility they assign to different information sources, their perceptions toward the current restrictiveness of animal welfare standards, and their socioeconomic characteristics.

Does the location of enrichment material affect behavior and dirtiness in growing female pigs?

The objective of this study was to investigate if female growing pigs spend similar amounts of time exploring a wooden stick as they do exploring enrichment material regardless of its proximity to the feeder. Forty-eight pigs aged 18 to 26 weeks allocated to 16 pens with three pigs per pen were studied. Fifty percent of the pens had a wooden stick beside the feeder, and the rest had a similar stick opposite to the feeder.

Effects of different rest-stop durations at control posts during a long journey on the welfare of sheep.

All livestock transport within the European Union must comply with the EC Regulation 1÷2005. For sheep, this law prescribes a maximum journey of 29 hours after which animals must rest in control posts (CP) for 24 hours before further transportation. However, there is no scientific evidence de ning the effects of di erent stop duration on sheep recovery during long journeys.

Can the way pigs are handled alter behavioural and physiological measures of affective state?

Research on human-animal relationship in animal production has been mainly focused on its effect on stress, productivity and meat quality. Only few studies have assessed its effects on the animals' affective state. In the present study, the influence of positive and negative handling (pH and NH, respectively) on affective state and fear as assessed by the cognitive bias test, the novel object test and the defence cascade test was studied in 56 pigs.