Effects of Ciprofloxacin Alone or in Mixture with Sulfamethoxazole on the Efficiency of Anaerobic Digestion and Its Microbial Community.

Some livestock farms rely on anaerobic digestion (AD) technology for manure disposal, thus obtaining energy (biogas) and fertilizer (digestate). Mixtures of antibiotics used for animal health often occur in organic waste and their possible synergistic/antagonistic effects on microorganisms involved in AD are still poorly studied. This work focuses on the effects of adding ciprofloxacin, alone (5 mg L) and in combination with sulfamethoxazole (2.

Bioaugmentation strategy to enhance polycyclic aromatic hydrocarbons anaerobic biodegradation in contaminated soils.

This paper proposes an innovative bioaugmentation approach for the remediation of polycyclic aromatic hydrocarbon (PAH) contaminated soils, based on a novel habitat-based strategy. This approach was tested using two inocula (i-24 and i-96) previously enriched through an anaerobic digestion process on wheat straw. It relies on the application of allochthonous microorganisms characterized by specific functional roles obtained by mimicking a natural hydrolytic environment such as the rumen.

Effects of Sulfamethoxazole on the Microbial Community Dynamics During the Anaerobic Digestion Process.

Anaerobic digestion (AD) treatment of cattle manure and slurry makes it possible to produce biogas, a renewable and storable biofuel, as well as digestate, a residual organic matter that can be used to replace chemical fertilizers. On the other hand, the intense use of antibiotics (e.g.

A novel enrichment approach for anaerobic digestion of lignocellulosic biomass: Process performance enhancement through an inoculum habitat selection.

Inocula enrichment was performed using an innovative habitat-based selection approach to improve wheat straw (WS) anaerobic digestion (AD) efficiency. The procedure was carried out by sequentially re-inoculating the primary microbial community seven times in subsequent anaerobic reactors containing untreated WS. Re-inocula were performed at different re-inoculum times (24, 48, and 96 h) by moving a porous support mimicking a rumen structure from one batch to the next (S-tests) or re-inoculating only the culture medium (C-tests).

A simplified model to simulate bioaugmented anaerobic digestion of lignocellulosic biomass: Biogas production efficiency related to microbiological data.

Mathematical model applications for the bioaugmented anaerobic digestion (BAD) process seem to be lacking in the scientific literature, even more so when related to microbiological data. The present study suggests a simplified mathematical model to investigate and simulate the process kinetics of bioaugmented anaerobic digestion (BAD) aimed at improving biogas production from wheat straw (WS). Bioaugmented conditions were obtained through a mixed inoculum of anaerobic ruminal fungi (ARF) and hydrogen-producing fermenting bacteria (F210) added to a methanogenic inoculum.

Combined bioaugmentation with anaerobic ruminal fungi and fermentative bacteria to enhance biogas production from wheat straw and mushroom spent straw.

Bioaugmentation with anaerobic ruminal fungi and a pool of hydrogen-producing fermenting bacteria was tested on wheat straw (WS) and mushroom spent straw (MSS) with the aim of improving anaerobic digestion performance. Batch tests were set up to simulate a Bioaugmentation Anaerobic Digestion (BAD) treatment comparing single- (I-BAD) and two-stage (II-BAD) process configurations, at two reactor scales, 120 and 1200 ml (×10). In both cases, higher CH cumulative production was obtained in the II-BAD configuration on WS (65.

Vertical distribution of bacterioplankton in Lake Averno in relation to water chemistry.

The effects of environmental factors on bacterioplankton distribution along the water column of Lake Averno (Naples, Italy) have been investigated by means of denaturing gradient gel electrophoresis (DGGE) of polymerase chain reaction (PCR)-amplified 16S rRNA gene fragments, and multivariate analysis applied to molecular data and physico-chemical parameters. Bacterial richness, estimated from DGGE profiles, remains constant throughout the water column, whereas archaeal richness increases with depth. Moreover, archaeal richness was significantly correlated to most of the measured abiotic variables, whereas bacterial richness did not.

The Paenibacillus polymyxa species is abundant among hydrogen-producing facultative anaerobic bacteria in Lake Averno sediment.

Lake Averno sediment was used to isolate the facultative anaerobic bacteria having the potential for H(2) production. Twenty-five out of 35 isolates recovered from the sediment sample produced hydrogen under anaerobic conditions from glucose with yields ranging from 0.1 to 0.