Micromachines (Basel)
September 2022
This article presents a new approach to determining the viscosity of Newtonian fluid. The approach is based on the analysis of the secondary Dean flow in a curved channel. The study of the flow patterns of water and aqueous solutions of glycerin in a microfluidic chip with a U-microchannel was carried out.
View Article and Find Full Text PDFThe rapid detection of pollutants in water can be performed with enzymatic probes, the catalytic light-emitting activity of which decreases in the presence of many types of pollutants. Herein, we present a microfluidic system for continuous chemoenzymatic biosensing that generates emulsion droplets containing two enzymes of the bacterial bioluminescent system (luciferase and NAD(P)H:FMN-oxidoreductase) with substrates required for the reaction. The developed chip generates "water-in-oil" emulsion droplets with a volume of 0.
View Article and Find Full Text PDFThe essential advantages of microfluidic studies are the excellent visualization of the processes of oil displacement from the porous medium model, simple cleaning, and the possibility of the repeated use of the microfluidic chip. The present article deals with the process of oil displacement by suspension flooding using a microfluidic chip, simulating a porous medium, and the suspensions of silicon dioxide nanoparticles (22 nm). The mass concentration of nanoparticles in suspensions ranged from 0.
View Article and Find Full Text PDFAdipose tissue is recognized as an important organ with metabolic, regulatory, and plastic roles. Adipose tissue-derived stem cells (ASCs) with self-renewal properties localize in the stromal vascular fraction (SVF) being present in a vascular niche, thereby, contributing to local regulation of angiogenesis and vessel remodeling. In the past decades, ASCs have attracted much attention from biologists and bioengineers, particularly, because of their multilineage differentiation potential, strong proliferation, and migration abilities and high resistance to oxidative stress and senescence.
View Article and Find Full Text PDFIn the present study, we demonstrate the use of a disposable luciferase-based microfluidic bioassay chip for environmental monitoring and methods for fabrication. The designed microfluidic system includes a chamber with immobilized enzymes of bioluminescent bacteria Photobacterium leiognathi and Vibrio fischeri and their substrates, which dissolve after the introduction of the water sample and thus activate bioluminescent reactions. Limits of detection for copper (II) sulfate, 1,3-dihydroxybenzene and 1,4-benzoquinone for the proposed microfluidic biosensor measured 3 μM, 15 mM, and 2 μM respectively, and these values are higher or close to the level of conventional environmental biosensors based on lyophilized bacteria.
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