Expression of guanylate cyclase C in human prefrontal cortex depends on sex and feeding status.

Introduction: Guanylate cyclase C (GC-C) has been detected in the rodent brain in neurons of the cerebral cortex, amygdala, midbrain, hypothalamus, and cerebellum.

Methods: In this study we determined GC-C protein expression in Brodmann areas (BA) 9, BA10, BA11, and BA32 of the human prefrontal cortex involved in regulation of feeding behavior, as well as in the cerebellar cortex, arcuate nucleus of hypothalamus and substantia nigra in brain samples of human 21 male and 13 female brains by ELISA with postmortem delay < 24 h.

Results: GC-C was found in all tested brain areas and it was expressed in neurons of the third cortical layer of BA9.

Postmortem injury quantification for the fatally injured cyclists in the Osijek-Baranja county over a 21-year period.

Objective: The objective of this study was to quantify injuries in fatal cyclists' traffic crashes by performing injury analysis and determine injury trends as well as main epidemiological data of the cyclists' fatalities.

Methods: The study was organized as a cross-sectional retrospective study, which included 125 cyclists' fatalities autopsied at the Clinical Department of Pathology and Forensic Medicine, Clinical Hospital Center Osijek from 1998 to 2018. We applied The Abbreviated Injury Scale (AIS©) classification for all fatally injured cyclists in traffic crashes and determined the severity of injuries by body region from the description of injuries reported in the autopsy report.

Fatal motorcycle crashes in wide urban area of Zagreb, Croatia-A 10-year review.

The objective of our study was to determine the prevalence of alcohol and drug intoxication among fatally injured motorcyclists in a wide urban area of Zagreb, Croatia. We conducted a single-center observational retrospective study over a 10-year period (2007-2016) in 3 counties covering an area including 1.2 million residents.