Publications by authors named "Antoinette Sero"
Article Synopsis
- mRNA therapies have become popular, especially after the approval of mRNA vaccines against SARS-CoV-2, but large-scale production faces significant challenges.
- The production process can be inefficient and costly due to issues with incorporating necessary cap analogs and the creation of unwanted double-stranded RNA byproducts that can trigger immune reactions.
- Researchers have developed a new RNA polymerase called T7-68 that improves the incorporation of cap analogs and reduces the presence of harmful dsRNA, enhancing the efficiency of mRNA production.
Fabry disease is caused by a deficiency of α-galactosidase A (GLA) leading to the lysosomal accumulation of globotriaosylceramide (Gb3) and other glycosphingolipids. Fabry patients experience significant damage to the heart, kidney, and blood vessels that can be fatal. Here we apply directed evolution to generate more stable GLA variants as potential next generation treatments for Fabry disease.
View Article and Find Full Text PDFInteractions between membrane proteins and the soluble fraction are essential for signal transduction and for regulating nutrient transport. To gain insights into the membrane-based interactome, 3,852 open reading frames (ORFs) out of a target list of 8,383 representing membrane and signaling proteins from Arabidopsis thaliana were cloned into a Gateway-compatible vector. The mating-based split ubiquitin system was used to screen for potential protein-protein interactions (pPPIs) among 490 Arabidopsis ORFs.
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