Scale-Up to Pilot of a Non-Axenic Culture of Thraustochytrids Using Digestate from Methanization as Nitrogen Source.

The production of non-fish based docosahexaenoic acid (DHA) for feed and food has become a critical need in our global context of over-fishing. The industrial-scale production of DHA-rich Thraustochytrids could be an alternative, if costs turned out to be competitive. In order to reduce production costs, this study addresses the feasibility of the non-axenic (non-sterile) cultivation of on industrial substrates (as nitrogen and mineral sources and glucose syrup as carbon and energy sources), and its scale-up from laboratory (250 mL) to 500 L cultures.

Changes in lipid and fatty acid contents of gonad during the reproductive cycle of the Mediterranean swordfish Xiphias gladius.

Swordfish Xiphias gladius is a large pelagic fish distributed worldwide and exploited for human consumption, however there is limited knowledge about its reproductive biology, especially regarding lipid dynamic in gonads. In teleost fish, reproductive success and offspring survival are associated to lipid availability for gamete synthesis. This study investigated the lipid composition, including lipid classes and fatty acids (FA) of cell membrane and reserve lipids (i.

A CO Enrichment Experiment to Study the Synthesis Pathways of Polyunsaturated Fatty Acids of the Haptophyte .

The production of polyunsaturated fatty acids (PUFA) in was studied using the gradual incorporation of a C-enriched isotopic marker, CO, for 24 h during the exponential growth of the algae. The C enrichment of eleven fatty acids was followed to understand the synthetic pathways the most likely to form the essential polyunsaturated fatty acids 20:5n-3 (EPA) and 22:6n-3 (DHA) in . The fatty acids 16:0, 18:1n-9 + 18:3n-3, 18:2n-6, and 22:5n-6 were the most enriched in C.

Risk and benefit assessment of seafood consumption harvested from the Pertuis Charentais region of France.

Seafood is well recognized as a major source of Long Chain n-3 Polyunsaturated Fatty Acids (LC n-3 PUFA, especially ecosapentaenoic acid, i.e. EPA and docosaheaxaenoic acid, i.

Post-mortem storage conditions and cooking methods affect long-chain omega-3 fatty acid content in Atlantic mackerel (Scomber scombrus).

Long-chain omega-3 fatty acids such as eicosapentaenoic (EPA) and docosahexaenoic acids (DHA) are health beneficial lipids found in high concentration in pelagic fishes, including Atlantic mackerel. While EPA and DHA are sensitive to oxidation during fish storage and processing, post-mortem degradation in the first hours following fish death is poorly documented. Here, we stored fish at two temperatures (2-4 °C and 18-20 °C) and monitored EPA + DHA content in dorsal fillet 6, 12 and 24 h after fish death and after cooking (grill or steam).

An Integrated Biomarker Approach Using Flounder to Improve Chemical Risk Assessments in the Heavily Polluted Seine Estuary.

The objective of this study was to develop an integrative approach in ecotoxicology (from biomarkers to population genetics) to assess the ecological status of fish populations. Flounders () collected after the spawning season in the heavily polluted Seine estuary were compared with the moderately polluted Bay of Douarnenez. The muscle energetic reserves were highly depleted in Seine vs.

Benefit-risk associated with the consumption of fish bycatch from tropical tuna fisheries.

Mercury, omega-3 (docosahexaenoic acid, DHA and eicosapentaenoic acid, EPA) and macronutrients (fat and proteins) were quantified on a wet weight (ww) basis in 20 species of fish taken as bycatch in tropical tuna fisheries. Based on a hazard quotient taking into account mercury and omega-3 contents, a benefit-risk assessment for the consumption of these pelagic species was conducted for three people categories: young children, children and adults. All fish bycatch were found to be an excellent source of proteins (min‒max = 14.

Identification of Polyunsaturated Fatty Acids Synthesis Pathways in the Toxic Dinophyte Using C-Labelling.

The synthetic pathways responsible for the production of the polyunsaturated fatty acids 22:6n-3 and 20:5n-3 were studied in the Dinophyte . The purpose of this work was to follow the progressive incorporation of an isotopic label (CO) into 11 fatty acids to better understand the fatty acid synthesis pathways in . The Dinophyte growth was monitored for 54 h using high-frequency sampling.

Study of Synthesis Pathways of the Essential Polyunsaturated Fatty Acid 20:5n-3 in the Diatom Using C-Isotope Labeling.

The present study sought to characterize the synthesis pathways producing the essential polyunsaturated fatty acid (PUFA) 20:5n-3 (EPA). For this, the incorporation of C was experimentally monitored into 10 fatty acids (FA) during the growth of the diatom for 24 h preferentially and quickly incorporated C into C PUFAs such as 18:2n-6 and 18:3n-6 as well as 16:0 and 16:1n-7, which were thus highly C-enriched. During the experiment, 20:5n-3 and 16:3n-4 were among the least-enriched fatty acids.

Nanoplastics exposure modulate lipid and pigment compositions in diatoms.

The impact of nanoplastics (NP) using model polystyrene nanoparticles amine functionalized (PS-NH) has been investigated on pigment and lipid compositions of the marine diatom Chaetoceros neogracile, at two growth phases using a low (0.05 μg mL) and a high (5 μg mL) concentrations for 96 h. Results evidenced an impact on pigment composition associated to the light-harvesting function and photoprotection mainly at exponential phase.

Trophic ecology of suspension-feeding bivalves inhabiting a north-eastern Pacific coastal lagoon: Comparison of different biomarkers.

Understanding the nature and origins of food sources supporting coastal lagoon-inhabiting organisms is necessary to evaluate the ecological status of such ecosystems. The trophic ecology of a bivalve species Spondylus crassisquama was studied in the Ojo de Liebre lagoon (Baja California, Mexico), combining stable isotope (SI), fatty acid (FA) and sterol analyses along a transect under oceanic influences. The second objective of the study was to investigate if sterol compositions give complementary information to those obtained from FA and SI.

Effects of extraction method and storage of dry tissue on marine lipids and fatty acids.

Various protocols are currently used to study marine lipids, but there is a growing interest in working on dry samples that are easier to transport. However, reference protocols are still lacking for dry samples. In order to make recommendations on this use, lipid classes and fatty acids (FA) obtained from six analytical protocols using two different tissue states (dry vs wet) and three extraction methods (automat vs manual potter vs leaving the solvent to work on tissue) were compared.