Methodology for processing mastectomy and cryopreservation of breast cancer tissue in a resource- poor setting: A pilot study.

Background: There is scarcity of breast cancer tissues derived from women of African origin available for patient - derived xenograft and organoid models.

Objective: We aim to create a versatile protocol for processing mastectomy and cryopreservation of breast cancer tissue.

Methodology: An immediate collection of breast cancer tissue from mastectomy was bathed in 4 °C HBSS and immediately transferred to 4 °C RPMI1640 containing HEPES, 10% FBS, Streptomycin and Penicillin.

Two logistic models for the prediction of hypothyroidism in pregnancy.

Background: The mounting evidence linking hypothyroidism during pregnancy with poor pregnancy outcome underscores the need for screening and, therefore, a search for more reliable and cheaper screening methods.

Methods: The study was conducted in two phases. The phase one study comprised of healthy women in different stages of pregnancy who attended routine antenatal clinic at St Theresa's Maternity Hospital, Enugu, Nigeria from September 6 to October 18 1994.