Publications by authors named "Anthony T Meger"
Article Synopsis
- The study explores how the function of proteins shapes their fitness landscapes, distinguishing between smooth landscapes (where small changes lead to gradual function variations) and rugged landscapes (where changes result in unpredictable function shifts).
- Through examining 1,158 sequences from the LacI/GalR transcriptional repressor family, the research found a rugged landscape with rapid changes in specificity between closely related sequences.
- The ruggedness of this landscape is linked to the need for the repressor to evolve a specific function while avoiding conflicting regulatory interactions, providing new insights into the evolution of genetic regulation.
Splitting bioactive proteins into conditionally reconstituting fragments is a powerful strategy for building tools to study and control biological systems. However, split proteins often exhibit a high propensity to reconstitute, even without the conditional trigger, limiting their utility. Current approaches for tuning reconstitution propensity are laborious, context-specific or often ineffective.
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