A Transcriptomics Analysis of the Regulation of Lens Fiber Cell Differentiation in the Absence of FGFRs and PTEN.

Adding 50% vitreous humor to the media surrounding lens explants induces fiber cell differentiation and a significant immune/inflammatory response. While loss blocks differentiation in lens epithelial explants, this blockage is partially reversed by deleting . To investigate the functions of the and during lens fiber cell differentiation, we utilized a lens epithelial explant system and conducted RNA sequencing on vitreous humor-exposed explants lacking , or or both and We found that loss impairs both vitreous-induced differentiation and inflammation while the additional loss of restores these responses.