Publications by authors named "Anthony Capelle"
Article Synopsis
- Developed and validated direct and indirect HPLC-UV methods for measuring anthraquinones in dried madder root.
- The direct method uses ethanol-water extraction to identify major glycosides, while the indirect method converts glycosides into aglycones before extraction.
- Indirect extraction is simpler and useful for determining key anthraquinones, such as alizarin and nordamnacanthal, with a proportional relationship to lucidin primeveroside levels.
- Various madder cultivars were analyzed for their anthraquinone content.
For the production of a commercially useful dye extract from madder, the glycoside ruberythric acid has to be hydrolysed to the aglycone alizarin which is the main dye component. An intrinsic problem is the simultaneous hydrolysis of the glycoside lucidin primeveroside to the unwanted mutagenic aglycone lucidin. Madder root was treated with strong acid, strong base or enzymes to convert ruberythric acid into alizarin and the anthraquinone compositions of the suspensions were analysed by HPLC.
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