Regulation of the hypertonic stress response by the 3' mRNA cleavage and polyadenylation complex.

Maintenance of osmotic homeostasis is one of the most aggressively defended homeostatic set points in physiology. One major mechanism of osmotic homeostasis involves the upregulation of proteins that catalyze the accumulation of solutes called organic osmolytes. To better understand how osmolyte accumulation proteins are regulated, we conducted a forward genetic screen in Caenorhabditis elegans for mutants with no induction of osmolyte biosynthesis gene expression (Nio mutants).

Alternative polyadenylation is a determinant of oncogenic Ras function.

Alternative polyadenylation of mRNA has important but poorly understood roles in development and cancer. Activating mutations in the Ras oncogene are common drivers of many human cancers. From a screen for enhancers of activated Ras () in , we identified , a subunit of the alternative polyadenylation machinery.

Stoichiometric and catalytic isomerization of alkenylboranes using bulky Lewis bases.

1,1-Carboboration of alkynes with boranes R'B(CF) (R' = (CH)Ph, CF) affords mixtures of the E and Z isomers of RCH = CR'B(CF) (R = (CH)Me, (CH)Me, (CH)Ph, CHCHMe, CHMe, CMe, CHOMe, CHN(CO)CH), treatment of these alkenylboranes with stoichiometric or catalytic amounts of tBuPH or other donors are shown to induce isomerization. The proposed mechanism involving a zwitterionic borataalkene intermediate is supported by computations.