miR-3191 promotes the proliferation and metastasis of hepatocellular carcinoma via regulating PAK6.

Background/aims: microRNAs (miRNAs) contribute to tumorigenesis, progression and drug resistance of hepatocellular carcinoma (HCC). miR-3191 is a newly discovered miRNA, and its function and mechanism of action in biological processes and diseases are not completely understood.

Methods: miR-3191 expression is determined via quantitative real-time polymerase chain reaction.

Characteristics, Outcomes and Factors for Place of Death in Patients Admitted to Community-Based Palliative Care Services in Shanghai China: A Multicenter Retrospective Cohort Study.

Background: Community-based palliative care (CBPC) is only available in large cities in mainland China and little is known about who utilizes it.

Objectives: This study examined the characteristics, outcomes, and factors associated with place of death (PoD) among inpatient CBPC patients.

Design: This was a multicenter retrospective cohort study.

Methyl Jasmonate (MeJA) Promotes the Self-Pollen Tube Growth of by Regulating Lignin Biosynthesis.

Self-incompatibility (SI) poses a significant reproductive barrier, severely impacting the yield, quality, and economic value of . In this study, methyl jasmonate (MeJA) was employed as an exogenous stimulus to alleviate SI in . The research findings revealed that an exogenous dose of 1000 μmol·L MeJA enhanced the germination and tube growth of self-pollen and greatly improved ovule penetration (18.

A Multifunctional Biomimetic Nanoplatform for Dual Tumor Targeting-Assisted Multimodal Therapy of Colon Cancer.

The biomimetic nanoparticles (NPs) possessing abilities of tumor targeting and multimodal therapy show great potential for efficient combat of colon cancer. Herein, we developed a multifunctional biomimetic nanoplatform (FeO@PDA@CaCO-ICG@CM) based on CaCO-modified magnetic polydopamine (PDA) loaded with indocyanine green (ICG), which was encapsulated by a mouse lymphoma cell (EL4) membrane (CM) expressing functional proteins (i.e.

Effects of slight shading in summer on the leaf senescence and endogenous hormone and polyamine contents in herbaceous peony.

Herbaceous peony is a perennial root plant that likes light and is cold-resistant. During summer, high temperature and strong light intensity advance its entry into the leaf wilting stage, which limits the accumulation of nutrients and formation of strong buds and severely affects its growth and development the following year. In this study, the wild herbaceous peony species and two main cultivars, 'Zifengyu' and 'Hongfengyu', were subjected to slight shading and strong light environments in summer, and their effects on leaf senescence and endogenous hormone and polyamine contents were explored.

[The number of TIGITCD8 T cells increases but their cytokine secretion decreases in the lungs of Plasmodium yoelii infected mice].

Objective To investigate the effect of T cell immunoreceptor with Ig and ITIM domains (TIGIT) on the function of CD8 T cells in the lungs of Plasmodium infected mice. Methods The lungs of the mice infected with Plasmodium yoelii were isolated, weighed and photographed after 12 days' infection. After dissolution, lung lymphocytes were isolated, counted and stained, and then the contents of CD8 and TIGITCD8 T cells were detected by flow cytometry.

Modified Prebiotic-Based "Shield" Armed Probiotics with Enhanced Resistance of Gastrointestinal Stresses and Prolonged Intestinal Retention for Synergistic Alleviation of Colitis.

Oral administration of probiotics is a promising method to alleviate inflammatory bowel diseases (IBDs). However, gastrointestinal environmental sensitivity and inferior intestinal colonization of probiotics hinder the alleviation effect. Here, we developed a simple yet effective modified prebiotic-based "shield" (Fe-TA@mGN) composed of an Fe-tannic acid cross-linking network and carboxymethylated β-glucan for arming Nissle 1917 (EcN@Fe-TA@mGN).

Protective Effect of Polysaccharide against Cyclophosphamide-Induced Immunosuppression in BALB/c Mice.

In this study, polysaccharide (AFP) was acquired a via water extraction and alcohol precipitation method. The immunoregulatory activity of AFP was first evaluated on cyclophosphamide (Cy)-treated mice. Galacturonic acid, glucose and galactose were confirmed to be the main components of AFP.

Characteristics of γδTCR on myeloid cells from C57BL/6 mice with Plasmodium yoelii nigeriensis infection.

Recently, there is a paucity of studies focus on the characteristics of myeloid cells which expressed γδTCR. The aim of this study was to observe the properties of γδTCR-expressing myeloid cells in the spleen of C57BL/6 mice infected by P. yoelii nigeriensis NSM.

Photo-controlled and photo-calibrated nanoparticle enabled nitric oxide release for anti-bacterial and anti-biofilm applications.

After numerous efforts to elucidate the biological role of nitric oxide (NO), NO treatments have become a hotspot at the forefront of medicine. NO-releasing substances are constantly needed, while the direct use of NO gas is unattainable in bio-systems. An ideal NO donor should possess controllable and visible NO-release capability.

TLR7 controls myeloid-derived suppressor cells expansion and function in the lung of C57BL6 mice infected with Schistosoma japonicum.

Toll-like receptors (TLRs) play an important role in the induction of innate and adaptive immune responses against Schistosoma japonicum (S. japonicum) infection. However, the role of Toll-like receptor 7 (TLR7) in the mouse lung during S.

Residue behavior and risk assessment of pyraclostrobin and tebuconazole in peppers under different growing conditions.

This study evaluates the residue behavior and risks of pyraclostrobin and tebuconazole in peppers. An analytical method for the simultaneous determination of the concentration of these fungicides in peppers was developed using ultra-high performance liquid chromatography-triple quadrupole mass spectrometry. Pepper samples were extracted with acetonitrile and cleaned with primary secondary amine and graphitized carbon black.

PD-1 CD4 T cell immune response is mediated by HIF-1α/NFATc1 pathway after infection.

The morbidity and mortality of malaria are still high. Programmed cell death-1(PD-1) is an important co-inhibitory factor and CD8 T cells with PD-1 were reported to be exhausted cells. It remains unknown what the role of CD4 T cells expressing PD-1 is and what the upstream regulating molecules of PD-1 in CD4 T cells are.

Properties and Roles of γδT Cells in NSM Infected C57BL/6 Mice.

Background: Many kinds of immune cells are involved in malaria infection. γδT cells represent a special type of immune cell between natural and adaptive immune cells that play critical roles in anti-parasite infection.

Methods: In this study, malaria infection model was constructed.

TLR7 modulating B-cell immune responses in the spleen of C57BL/6 mice infected with Schistosoma japonicum.

B cells played an important role in Schistosoma infection-induced diseases. TLR7 is an intracellular member of the innate immune receptor. The role of TLR7 on B cells mediated immune response is still unclear.

Ikzf2 Regulates the Development of ICOS Th Cells to Mediate Immune Response in the Spleen of -Infected C57BL/6 Mice.

Background: Th cells (helper T cells) have multiple functions in () infection. Inducible co-stimulator (ICOS) is induced and expressed in activated T lymphocytes, which enhances the development of B cells and antibody production through the ICOS/ICOSL pathway. It remains unclear about the role and possible regulating mechanism of ICOS Th cells in the spleen of -infected C57BL/6 mice.

Interferon Regulatory Factor 4 Regulates the Development of Polymorphonuclear Myeloid-Derived Suppressor Cells Through the Transcription of c-Myc in Cancer.

The accumulation of myeloid-derived suppressor cells (MDSCs) is one of the major obstacles to achieve an appropriate anti-tumor immune response and successful tumor immunotherapy. MDSCs in tumor-bearing hosts are primarily polymorphonuclear (PMN-MDSCs). However, the mechanisms regulating the development of MDSCs remain poorly understood.

Purification, structure and conformation characterization of a novel glucogalactan from Anoectochilus roxburghii.

Anoectochilus roxburghii (AR) has been used in food, medicine and ornamental industries for a long time. Anion exchange resin was proposed to purify the sub-fraction of water-extracted AR polysaccharide (ARPP-70), and a homogeneous polysaccharide ARPP-70a was obtained. The structural features of ARPP-70a were characterized using gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR) spectroscopy, and high performance size exclusion chromatograph coupled with multi-angle laser light scattering (HPSEC-MALLS).

Adjustments of γδ T Cells in the Lung of -Infected C56BL/6 Mice.

Many kinds of lymphocytes are involved in () infection-induced disease. γδ T cells comprise a small number of innate lymphocytes that quickly respond to foreign materials. In this study, the role of γδ T cells in the lung of -infected C56BL/6 mice was investigated.

Hepatic CXCL16 is increased in gallstone accompanied with liver injury.

Background And Aims: This study aimed to investigate the relationship between circulating soluble C-X-C chemokine ligand 16 (CXCL16) levels and clinical characteristics of gallstone.

Methods: 93 subjects including 53 subjects with gallstone, 25 subjects with nonalcoholic fatty liver disease (NAFLD), and 40 control subjects were recruited. All gallstone subjects underwent ultrasounds to confirm the gallstone patients.

CXCL16 deficiency attenuates acetaminophen-induced hepatotoxicity through decreasing hepatic oxidative stress and inflammation in mice.

Chemokine C-X-C ligand 16 (CXCL16), a single-pass Type I membrane protein belonging to the CXC chemokine family, is related to the inflammatory response in liver injury. In present study, we investigated the pathophysiological role of CXCL16, a unique membrane-bound chemokine, in acetaminophen (APAP)-induced hepatotoxicity in mice. Mice were injected with APAP, and blood and tissue samples were harvested at different time points.