ZmHSFA2B self-regulatory loop is critical for heat tolerance in maize.

The growth and development of maize (Zea mays L.) are significantly impeded by prolonged exposure to high temperatures. Heat stress transcription factors (HSFs) play crucial roles in enabling plants to detect and respond to elevated temperatures.

Ferroptosis MRI for early detection of anticancer drug-induced acute cardiac/kidney injuries.

Ferroptosis has been realized in anticancer drug-induced acute cardiac/kidney injuries (ACI/AKI); however, molecular imaging approach to detect ferroptosis in ACI/AKI is a challenge. We report an artemisinin-based probe (Art-Gd) for contrast-enhanced magnetic resonance imaging of ferroptosis (feMRI) by exploiting the redox-active Fe(II) as a vivid chemical target. In vivo, the Art-Gd probe showed great feasibility in early diagnosis of anticancer drug-induced ACI/AKI, which was at least 24 and 48 hours earlier than the standard clinical assays for assessing ACI and AKI, respectively.

Identification and Analysis of Stress-Associated Proteins (SAPs) Protein Family and Drought Tolerance of in Transgenic .

Stress-associated proteins (SAPs), a class of A20/AN1 zinc finger proteins, play vital roles in plant stress response. However, investigation of SAPs in maize has been very limited. Herein, to better trace the evolutionary history of SAPs in maize and plants, 415 SAPs were identified in 33 plant species and four species of other kingdoms.

ZmmiR190 and its target regulate plant responses to drought stress through an ABA-dependent pathway.

MicroRNAs (miRNAs) are small, non-coding regulatory RNAs that regulate gene expression by facilitating target mRNA cleavage in plants. They are crucial for responses to diverse stresses. The novel drought-responsive miRNA ZmmiR190 was previously identified during an analysis of the maize transcriptome.

Towards next-generation model microorganism chassis for biomanufacturing.

Synthetic biology provides powerful tools and novel strategies for designing and modifying microorganisms to function as cell factories for biomanufacturing, which is a promising approach for realizing chemical production in a green and sustainable manner. Recent advances in genetic component design and genome engineering have enabled significant progresses in the field of synthetic biology chassis that have been developed for enzymes or biochemical production based on synthetic biology strategies, with particular reference to model microorganisms, such as Escherichia coli, Bacillus subtilis, Corynebacterium glutamicum, and Saccharomyces cerevisiae. In this review, strategies for engineering four different functional cellular modules which encompass the total process of biomanufacturing are discussed, including expanding the substrate spectrum for substrate uptake modules, refactoring biosynthetic pathways and dynamic regulation for product synthesis modules, balancing energy and redox modules, and cell membrane and cell wall engineering of product storage and secretion modules.

Developing rapid growing for improved biochemical and recombinant protein production.

is a model Gram-positive bacterium, which has been widely used as industrially important chassis in synthetic biology and metabolic engineering. Rapid growth of chassis is beneficial for shortening the fermentation period and enhancing production of target product. However, engineered with faster growth phenotype is lacking.

Enhanced removal of Cr(VI) by silicon rich biochar-supported nanoscale zero-valent iron.

Silicon-rich biochar-supported nanoscale zero-valent iron (nZVI) was studied to evaluate enhanced removal of hexavalent chromium (Cr(VI)) in solution. The compositional structures of the nZVI and biochar-supported nZVI were analyzed by Fourier transform infrared spectroscopy, X-ray diffraction and X-ray photoelectron spectra before and after Cr(VI) reaction. The removal amount of Cr(VI) by nZVI-RS700 (rice straw pyrolyzed at 700 °C) was considerably greater than that by nZVI and other biochar-supported nZVI samples.

Effect of culturing temperatures on cadmium phytotoxicity alleviation by biochar.

Biochar produced from rice straw at 400 °C (RS400) was prepared to determine its alleviating effect on Cd phytotoxicity to wheat seedlings under different cultivation temperatures and pH. A hydroponic system (pH 4.3) and a loam soil slurry system were designed to respectively simulate acidic and neutral soil condition, and cultivation at increasing temperatures (20, 25, and 30 °C) were performed to evaluate the greenhouse effect.

Degradation of 1,4-dioxane by biochar supported nano magnetite particles activating persulfate.

Nano magnetite biochar composite (nFeO/biochar) was synthesized and used to activate persulfate for the degradation of 1,4-dioxane. Analytical techniques using X-ray diffraction (XRD), fourier transform infrared analysis (FTIR), X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM) indicated that nFeO was spherical and successfully loaded onto the surface of biochar. The results of batch-scale experiments illustrated that the 1,4-dioxane degradation efficiency in aqueous phase was 98.