Publications by authors named "Anosha Siripala"
Article Synopsis
- There's a big problem in how scientists describe and share information about tests for new drugs, which makes it hard for them to use data effectively.
- To solve this, they've created something called the BioAssay Ontology (BAO) to help standardize terms and definitions for these tests, making it easier to analyze the data.
- BAO has been improved since it started in 2010, and now it can model different types of tests while allowing researchers to share and reuse parts of the ontology in different projects without confusion.
Many bacterial pathogens reorganize the host actin cytoskeleton during the course of infection, including enterohemorrhagic Escherichia coli (EHEC), which utilizes the effector protein EspF(U) to assemble actin filaments within plasma membrane protrusions called pedestals. EspF(U) activates N-WASP, a host actin nucleation-promoting factor that is normally auto-inhibited and found in a complex with the actin-binding protein WIP. Under native conditions, this N-WASP/WIP complex is activated by the small GTPase Cdc42 in concert with several different SH3 (Src-homology-3) domain-containing proteins.
View Article and Find Full Text PDFEnterohemorrhagic Escherichia coli (EHEC) generate F-actin-rich adhesion pedestals by delivering effector proteins into mammalian cells. These effectors include the translocated receptor Tir, along with EspF(U), a protein that associates indirectly with Tir and contains multiple peptide repeats that stimulate actin polymerization. In vitro, the EspF(U) repeat region is capable of binding and activating recombinant derivatives of N-WASP, a host actin nucleation-promoting factor.
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