Antiretroviral activity of (red lionfish) venom in the early development of human immunodeficiency virus/acquired immunodeficiency syndrome antiretroviral alternative source.

Aim: This study aimed to investigate the antiviral activity of phospholipase A2 (PV-PLA2) from Indonesia to human immunodeficiency virus (HIV).

Materials And Methods: Fresh venomous fin parts of wild PV specimens were collected from Java Sea waters. Then, it washed using phosphate buffer pH 7.

Antiviral activity of phospholipase A2 against human immunodeficiency virus.

Aim: Investigation of antiviral activity of phospholipase A2 (AP-PLA2) from moluccas to human immunodeficiency virus (HIV).

Materials And Methods: Crude venom (CV) and F20 (PLA2 with 20% fractioned by ammonium sulfate) as a sample of PLA 2 obtained from extract were used. Enzymatic activity of PLA2 was determined using the degradation of phosphatidylcholine (PC).

Rigorous kinetic model considering positional specificity of lipase for enzymatic stepwise hydrolysis of triolein in biphasic oil-water system.

A rigorous kinetic model describing the stepwise triglyceride hydrolysis at the oil-water interface, based on the Ping Pong Bi Bi mechanism using suspended lipase having positional specificity, was constructed. The preference of the enzyme to cleave to the ester bonds at the edge and the center of the glycerol backbone of the substrates (tri-, di- or monoglyceride) was incorporated in the model. This model was applied to the experimental results for triolein hydrolysis using suspended Porcine pancreatic lipase (an sn-1,3 specific lipase) and Candida rugosa lipase (a non-specific lipase) in a biphasic oil-water system under various operating conditions.