Biochemical and Epigenetic Regulation of Glutamate Metabolism in Maize ( L.) Leaves under Salt Stress.

The effect of salt stress (150 mM NaCl) on the expression of genes, methylation of their promoters, and enzymatic activity of glutamate dehydrogenase (GDH), glutamate decarboxylase (GAD), and the 2-oxoglutarate (2-OG)-dehydrogenase (2-OGDH) complex was studied in maize ( L.). GDH activity increased continuously under salt stress, being 3-fold higher after 24 h.

Effects of light, anoxia and salinity on the expression of dihydroxyacid dehydratase in maize.

Dihydroxyacid dehydratase (EC 4.2.1.

[Magnetic resonance relaxometry of serum in patients with different forms of cellulitis in the dynamics of the disease].

Aim: To study the time of nuclear magnetic resonance (NMR) of spin-lattice serum relaxation (T1) in the dynamics of an infectious process in patients with different forms of erysipelas.

Materials And Methods: 37 patients with different clinical forms of erysipelas were examined in the acute period of the disease and in the early period of convalescence.

Results: A statistically significant increase in T1 NMR was ascertained in patients with bullous hemorrhagic erysipelas as compared with those with erythematous form.

[Duphalac in combination therapy of patients with hepatic cirrhosis].

The article presents findings from examination of 15 patients with cirrhosis of the liver complicated by encephalopathy. A study was made into the blood content of aminoacids and ammonia, which levels were found to have been raised in cirrhotic patients. The use of dufalac and nutriklinz dispelled clinical manifestations of encephalopathy normalizing the content of ammonia and modifying the aminoacid dysbalance at the expense of lowering of the blood concentration of cerebrotoxic aminoacids.

[The enzyme preparation Kirshner Pancreal in the treatment of patients with chronic pancreatitis].

Our objective in this study was investigation into the effectiveness of the enzymic preparation Kirshner Pancreal in 27 patients with chronic pancreatitis. It is shown that against the background of treatment with the above drug patients dispelled the pain syndrome and manifestations of the maldigestion syndrome. The described drug made for normalization of the blood serum content of beta-lipoproteins.

[The use of Smecta for the treatment of the basic digestive tract diseases].

The indices for the gastric contents aggression and protection ratio returned to normal in patients with peptic ulcer and preulcerous condition during the treatment with smekta due to a decrease in proteolytic activity and increase in the synthesis of the mucus protective proteins. Monitoring of intragastric pH in the body of the stomach throughout 24 hours in patients with peptic ulcer of duodenal location revealed significant acid-neutralizing effect of smekta. Patients with nonspecific ulcerous colitis enjoyed freedom of side effects as a result of the use of salofalk and derived full therapeutic benefit from this treatment.

[A case of seronegative systemic lupus erythematosus (an immunopathological study)].

A case of systemic lupus erythematosus (SLE) in a 37-year-old female is described which was primarily diagnosed as epilepsy. Immunopathologically, an immunoglobulin in the nuclei of the majority of cells in the skin biopsy and blood cells was found against the background of the lack of SLE manifestations (antinuclear factor in the circulating blood, skin lesions, etc.).

[Changes in the phospholipid and prostaglandin levels in chronic diseases of the biliary tract].

A study of 179 patients with functional and organic diseases of the bile tract revealed that changes of the blood phospholipids and their spectra in the blood is observed at more early stages of the disease, functional pathology and that an increased PGE2/PGF2 indicates a deficit of polyunsaturated fatty acids and prevalence of the processes of break-up of prostaglandins over their synthesis.

[The immunopathological mechanisms of the skin lesion in erysipelas patients].

The localization of bound IgG, IgA, IgM and component C3 of the complement in the focus of skin lesion in patients with different forms of erysipelas has been studied by the method of direct immunofluorescence. The presence of fixed immune complexes and component C3 in the affected areas of the skin at the acute period of the disease and their absence in the healthy areas of the skin have been established, which seems to be indicative of the presence of the local immunocomplex process in erysipelas. This process is one of the mechanisms of erysipelatous inflammation.

[Clinical and ultrasonic diagnosis of diseases of the hepatobiliary system in chronic alcoholics].

Functional and structural changes of the hepato-biliary system were assessed in 56 persons with different forms of chronic alcoholism. In patients with prenosological forms of chronic alcoholism despite the absence of clinical manifestations and functional disorders of the liver, phenomena of fatty infiltration of the liver were revealed by means of ultrasound examination. Patients with chronic alcoholism of grade II and III showed an increase of the activity of aminotransferases, increased values of the thymol test, hypoalbuminemia, disorders of lipid metabolism.

[Cholate-forming function of the liver in patients with chronic alcoholism].

The level of bile acids was studied in the bile and feces in 119 patients with chronic alcoholism using thin-layer chromatography. The cholate-forming function of the liver was found to increase in patients with short alcohol anamnesis in chronic alcoholism. With advance of the alcohol anamnesis the number of patients with reduced cholate-forming function of the liver increased.

[Cholate-forming and cholesterol-secreting function of the liver in patients with ulcers after vagotomy].

The cholate-forming and cholesterol-excretory function of the liver was studied in patients with ulcer disease of the duodenum after different types of vagotomy--selective proximal vagotomy and selective proximal vagotomy with pyloroplasty. The patients revealed different degrees of disorders in the content of bile acids, their fractions, bile cholesterol as well as a dependence of these changes of the type of operation. The changes were more pronounced in patients undergoing selective proximal vagotomy as compared with those undergoing selective proximal vagotomy with pyloroplasty.

[Significance of biochemical examination of the bile in the early diagnosis of chronic diseases of the biliary tract].

Three groups of subjects were examined without complaints characteristic of involvement of the hepatobiliary system. In 11% of healthy persons disorders of the biochemical composition of bile were revealed, namely reduction of the level of bile acids and phospholipids, 23% changes in the ratio of bile acids and phospholipid fractions.

[Pathogenesis of lipid metabolism disorders in patients with atherosclerosis of the vessels of the lower limbs].

The bile secretory function of the liver in patients with hyperlipoproteinemia complicated by atherosclerosis of the vessels of the lower extremities was studied. It was established, that irrespective of the type of hyperlipoproteinemia in patients, the impairement in the cholate-forming and cholesterin-excreting functions of the liver was present. This aggravated the course of atherosclerosis.

[Determination of antibodies against ribosomes and various cell wall components and detection of circulating antigens of group A Streptococcus in patients with erysipelas].

The level of antibodies to the ribosomes, polysaccharide A and peptidoglycan of group A streptococcus in the blood of patients with primary, secondary, and often relapsing erysipelas was studied by means of the enzyme immunoassay with the use of the sandwich techniques. For control, the sera of healthy donors were used. In the sera obtained from all groups of erysipelas patients a significant rise in the levels of antibodies to ribosomes and peptidoglycan in comparison with the controls was revealed.

[Determination of antibodies to Streptococcus group A polysaccharide in human sera by an immunoenzyme method].

Use was made of the ELISA to develop a highly sensitive quantitative method for detection of antibodies against Streptococcus group A polysaccharide (polysaccharide A) in human sera. The main advantage is that one can use only one optimal dilution of the sera together with the reference serum. Sera of 53 healthy volunteers and 77 patients with a history of Streptococcus group A infections were screened for the presence of polysaccharide A antibodies.