Publications by authors named "Anniko M"

The presence of intermediate filaments in the inner ear of the newborn mouse was analyzed with immunofluorescence techniques using antibodies against the five classes of intermediate filaments: cytokeratins, vimentin, desmin, neurofilaments and glial fibrillary acid protein (GFA). Neurofilaments were found in all nerve fibers from the ganglion cell to the hair cell. In the vestibular ganglion two subpopulations of ganglion cells were identified: a minor part staining intensively with neurofilament and the major part of cells lacking this immunofluorescence.

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Organ culture of the embryonic inner ear of the mouse results, under normal conditions, in a good morphological morphogenesis and cytodifferentiation of individual cells and tissues. Organ culture is performed during a period corresponding to the embryonic development in vivo. Continuing the culture period causes a deterioration of the organ culture explant.

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The freeze fracture technique was used to study intercellular junctions of inner ear anlages developed in vitro. The 16th gestational day inner ear from the CBA/CBA mouse was cultured for 5 days whereafter the specimens were analyzed. Inner ears developed in vivo were used as controls.

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ACTH-producing pituitary adenomas obtained from two patients with Cushing's disease were maintained in organ culture for 2 weeks. After 4-6 days of incubation, hydrocortisone (0.1, 1, and 5 mg/ml) was added to the culture medium.

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The 13th and 16th gestational day inner ear anlagen, respectively, were exposed after explantation to an organ culture system to low-dose gamma irradiation with a 2 Gy single dose. The explants were thereafter cultured in vitro for 8 vs. 5 days to an age corresponding to birth in vivo.

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Thirty-two CBA/CBA mice were irradiated in utero on the 12th, 13th, or 16th gestational day with doses of 0.5, 1, and 2 Gy, respectively (1 Gy = 100 rads). One month after birth, the inner ears were examined by light microscopy and transmission and scanning electron microscopy.

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The tumor tissue from 62 consecutive patients with pituitary adenomas was analyzed with regard to ploidy and percentage of cells in different phases of the cell cycle by use of flow-cytofluorometry. In six of 62 cases, two tumor-cell populations were identified; therefore the study material comprised 68 cell lines. Approximately half of the cell lines were diploid (33 of 68, or 49 per cent); five of 68 (7 per cent) were hypodiploid, and 30 of 68 (44 per cent) were hyperdiploid-aneuploid.

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The 12th-12.5th gestational day inner ear otocyst from the CBA/CBA mouse was explanted to organ culture with and without surrounding mesenchyme. One group of otocysts from which the mesenchyme had been removed was cultured in conditioned medium (i.

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An X-ray microanalytical and morphological investigation has been carried out on rapidly frozen, freeze-dried or freeze-substituted tissues. A comparison was made between different embedding and polymerisation procedures following freeze-substitution and freeze-drying. The investigation also included an analysis of specimens infiltrated, embedded and polymerised by ultraviolet irradiation at low temperatures with Lowicryl HM20.

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Surface pathology of inner ear structures so far described in detail concern cochlear and vestibular hair cells and the stria vascularis. In man, surgical intervention into the inner ear is very uncommon and when performed is in general with the primary objective of destroying the diseased peripheral end organs. The vast majority of inner ear tissue available for use with scanning electron microscopy (SEM) is therefore obtained from animals.

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Formation and maturation of the vestibular ganglion.

ORL J Otorhinolaryngol Relat Spec

April 1985

The morphologic development of the vestibular ganglia with their peripheral neuronal connections was followed from terminal mitosis until the adult stage (CBA/CBA mouse). Schwann cells enclose vestibular ganglion cells and their peripheral nerve fibres already on the 15th-16th gestational days. The morphological basis for at least some physiological activity is present already before birth.

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An 18-year-old patient with Gorlin's syndrome is described, and the clinical findings associated with the syndrome are discussed. The management of keratocysts occurring in patients with the syndrome requires a follow-up period of more than 5 years.

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Principles in cochlear toxicity.

Arch Toxicol Suppl

April 1986

The hair cells of the cochlea (neuroepithelium) represent the primary target in most drug-induced ototoxic adverse effects on hearing (e.g. aminoglycoside antibiotics).

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Microprobe analysis was performed at the cellular and subcellular levels of type I and type II vestibular hair cells. In principle the same types of elemental histograms were found in the two types of hair cells studied. High concentrations of Cl and K were detected in stereocilia, whereas calcium was found when analyzing stereocilia and the supranuclear cytoplasm.

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The developing inner ears of mice (CBA/CBA), ages ranging from gestational day 12 through postnatal day 21, were examined using scanning electron microscopy following the epoxy-embedding/freeze-fracture technique. This technique provides unique three-dimensional views of surface and fractured structures of the developing inner ear, thus allowing excellent preservation of the relationships between the developing sensory epithelium and the overlying membranes (i.e.

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The structure and function of the cochlea of the CBA/CBA mouse were analysed after exposure to single doses of gamma irradiation during embryonic development. Pregnant mice were exposed to 0.5, 1 and 2 Gy, respectively, on either the 12th, 13th, or 16th gestational day.

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Cochlear development has been studied by means of biochemistry/histochemistry (Na+/K+-ATPase, adenylate cyclase, 2-deoxy-D-glucose and phospholipids), the energy dispersive X-ray microanalysis technique and organ culture of the mammalian inner ear. A high level of Na+/K+-ATPase and adenylate cyclase occurs in the stria vascularis and has been cytochemically demonstrated at the contraluminal side of marginal cell membranes. An increase in the adenylate cyclase content occurs approximately one day before and in parallel with the rise of the potassium content in endolymph.

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Localization of elements at the cellular and sub-cellular levels was performed with the energy dispersive X-ray microanalysis technique, using shock-frozen, freeze-dried and araldite-embedded mouse (CBA/CBA) cochleae sectioned dry. Anatomical identification occurred in the STEM (scanning transmission electron microscopy) mode. In inner hair cell stereocilia the K/Na ratio was 70:1 but only 20:1 in the cytoplasm.

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The early embryonic development of the inner ear anlage, from the otic placode stage to formation of the otocyst, was documented in CBA/CBA mice by scanning electron microscopy. The otic placode is identified at the eight- to ten-somite stage, as revealed by the surface morphology of the developing ectoderm. The cells in the otic depression are considerably smaller than those of the adjacent ectodermal surface.

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Pregnant CBA/CBA mice (total gestational age 20-21 days) were whole-body irradiated on the 12th, 13th and 16th gestational days with single doses of 1 and 2 Gy using a 60Co source. Litters were taken for morphological analysis 14-21 days after birth. Irradiation on the 12th and 13th gestational days was thus performed prior to morphologic inner ear organogenesis and cytodifferentiation.

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[The in vitro system in internal ear research].

Laryngol Rhinol Otol (Stuttg)

August 1984

The knowledge about in vitro culture of the embryonic inner ear dates back to the 1920's. During the following decades it was mainly the avian embryonic labyrinth which was used for organ culture. Emphasis was put on histological differentiation of individual cells and tissues.

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Thin (130-200 nm) and thick (10-16 micrometers) cryosections of stapedius muscle of guinea pig were examined by X-ray microanalysis in the scanning- and scanning-transmission mode of electron microscopy. To further explore the unusual elemental composition of stapedius muscle and to evaluate previously used method of analysis of thick cryosections cut at -30 degrees C (Wróblewski et al., 1981), thin cryosections cut at -100 degrees C were investigated.

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The flow-cytofluorometric technique for nuclear DNA analysis was used in the study of 47 consecutive cases with pituitary tumors. The material consisted of 18 tumors secreting growth hormone (GH), 10 secreting GH and prolactin (PRL), 10 prolactinomas, 2 secreting thyroid-stimulating hormone (TSH), 2 secreting ACTH, and 5 were nonsecreting. An aneuploid DNA pattern occurred in 23 of 47 (49%) cases ranging from 1.

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Utricular and saccular otoconia (N = 510) from 1 fetus (39th gestational week; Trisomy-18), 2 babies (1 1/2 and 2 months) and 3 adults (58, 61 and 77 years) were analysed with an energy dispersive X-ray microprobe. In all otoconia the elemental composition showed an extremely high concentration of Ca but also Na, Mg, P, S, Cl and K were identified. The presence of non-Ca elements was interpreted as being due to a protein nucleus of the otoconia.

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Inductive tissue interactions were studied in the otocyst of the CBA/CBA mouse. Otocysts with surrounding mesenchyme explanted at the 12.5-13th gestational day and cultured in vitro for 4 days underwent morphogenesis with formation of semicircular canals, vestibular organs, and some cochlear coiling.

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