Publications by authors named "Annie Frazier"
Human rotavirus (HRV) is still a leading cause of severe dehydrating gastroenteritis globally, particularly in infants and children. Previously, we demonstrated the immunogenicity of mRNA-based HRV vaccine candidates expressing the viral spike protein VP8* in rodent models. In the present study, we assessed the immunogenicity and protective efficacy of two mRNA-based HRV trivalent vaccine candidates, encoding VP8* of the genotypes P[8], P[6], or P[4], in the gnotobiotic (Gn) pig model of Wa (G1P[8]) HRV infection and diarrhea.
View Article and Find Full Text PDFArticle Synopsis
- Human rotavirus (HRV) is a major cause of severe gastroenteritis in children, primarily affecting the gastrointestinal tract and causing symptoms like diarrhea and vomiting.
- Recent studies suggest that HRV might also infect salivary glands, similar to murine strains that transmit the virus through saliva between mother and pups.
- In a study using gnotobiotic pigs, researchers found that HRV was present in saliva, feces, and various tissues, indicating the virus can replicate in salivary tissues and trigger immune responses in both intestinal and facial lymphoid areas.
Combined Live Oral Priming and Intramuscular Boosting Regimen with Rotarix and a Nanoparticle-Based Trivalent Rotavirus Vaccine Evaluated in Gnotobiotic Pig Models of G4P[6] and G1P[8] Human Rotavirus Infection.
Vaccines (Basel)
May 2023
Article Synopsis
- Human rotavirus (HRV) is a leading cause of severe diarrhea in young children, particularly in low-income countries, where vaccine effectiveness is hampered by malnutrition and co-infections.
- A new two-dose intramuscular (IM) nanoparticle-based HRV vaccine was tested, showing good antibody responses but not significant protection against diarrhea; however, it did reduce the duration of virus shedding in pigs.
- Combining an oral vaccine with the IM nanoparticle vaccine improved immune responses, indicating the potential of this prime-boost strategy for better HRV prevention.
Human rotavirus (HRV) is a leading cause of gastroenteritis in children under 5 years of age. Licensed vaccines containing G1P[8] and G1-4P[8] strains are less efficacious against newly emerging P[6] strains, indicating an urgent need for better cross protective vaccines. Here, we report our development of a new gnotobiotic (Gn) pig model of P[6] HRV infection and disease as a tool for evaluating potential vaccine candidates.
View Article and Find Full Text PDFClostridioides difficile (C. difficile) is a gram-positive, spore-forming, anaerobic bacterium known to be the most common cause of hospital-acquired and antibiotic-associated diarrhea. C.
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- - The study aimed to assess if model-based iterative reconstruction (MBIR) enhances diagnostic confidence and detection rates of pulmonary embolism (PE) during computed tomography pulmonary angiography, compared to hybrid iterative reconstruction (HIR) and filtered back projection (FBP) techniques.
- - Fifty patients were evaluated, with each study reconstructed using FBP, HIR, and MBIR, and assessed by radiologists on various criteria, including diagnostic confidence and detection of PE, with results showing MBIR significantly improved PE detection and confidence levels compared to FBP and HIR.
- - The study found that while accuracy rates were similar across the three methods, MBIR had a notably higher sensitivity for detecting PE, as well as subjective improvements in image