Up-regulated DSG2 promotes tumor growth and reduces immune infiltration in cervical cancer.

Background: Desmoglein-2 (DSG2) has been reported to play pivotal roles in various diseases. However, its roles in cervical cancer (CC) remain insufficiently elucidated. Here, we aimed to comprehensively explore the functional mechanisms of DSG2 in CC using bioinformatics and experimental methods.

Enhancing Biocatalysis through Chiral Supramolecular Scaffolds: Insights into the Structural Adaptability of Lipase.

How to maintain high catalytic activity and stability in the process of biocatalysis is crucial, inspiring strategies to construct an appropriate catalytic microenvironment. Considering the lipase's inherent chirality and the necessity for a delicate hydrophilic-hydrophobic equilibrium, we crafted a chiral, nonaqueous catalytic microenvironment via the coassembly of Boc-FF-NHNH (Bfl) and lipase. Benefiting from the chirality and distinct Bfl-lipase interactions, the lipase@Bfl supramolecular hybrid amplifies biological functionalities and can serve as a versatile and highly efficient catalyst.

DHCR7 promotes lymph node metastasis in cervical cancer through cholesterol reprogramming-mediated activation of the KANK4/PI3K/AKT axis and VEGF-C secretion.

Cervical cancer (CC) patients with lymph node metastasis (LNM) have a poor prognosis. However, the molecular mechanism of LNM in CC is unclear, and there is no effective clinical treatment. Here, we found that 7-dehydrocholesterol reductase (DHCR7), an enzyme that catalyzes the last step of cholesterol synthesis, was upregulated in CC and closely related to LNM.

Establishment of a high-fidelity patient-derived xenograft model for cervical cancer enables the evaluation of patient's response to conventional and novel therapies.

Background: Recurrent or metastatic cervical cancer (r/m CC) often has poor prognosis owing to its limited treatment options. The development of novel therapeutic strategies has been hindered by the lack of preclinical models that accurately reflect the biological and genomic heterogeneity of cervical cancer (CC). Herein, we aimed to establish a large patient-derived xenograft (PDX) biobank for CC, evaluate the consistency of the biologic indicators between PDX and primary tumor tissues of patients, and explore its utility for assessing patient's response to conventional and novel therapies.

PIBF1 regulates multiple gene expression via impeding long-range chromatin interaction to drive the malignant transformation of HPV16 integration epithelial cells.

Introduction: Human papillomavirus (HPV) integration can induce gene expression dysregulation by destroying higher-order chromatin structure in cervical cancer.

Objectives: We established a 13q22 site-specific HPV16 gene knock-in cell model to interrogate the changes in chromatin structure at the initial stages of host cell malignant transformation.

Methods: We designed a CRISPR-Cas9 system with sgRNA targeting 13q22 site and constructed the HPV16 gene donor.

LncRNA HCP5 enhances the proliferation and migration of cervical cancer via miR-216a-5p/CDC42 axis.

To investigate the important roles of the cancer-promoting long non-coding RNAs (lncRNAs) in cervical cancer, the up-regulated lncRNAs and prognostic analysis were identified through Lnc2Cancer and Lncar. LncRNA-regulated miRNA and miRNA-target mRNA were analyzed based on starBase v2.0 and miTarbase to predict the lncRNA-miRNA-mRNA ceRNA network.

Systematic analysis of the long noncoding RNA (lncRNA)-miRNA-mRNA competing endogenous RNA network to identify prognostic biomarkers and the potential regulatory axis in glioblastoma multiforme.

Background: Glioblastoma (GBM) is an intracranial brain tumor characterized by a high final lethality rate and recurrence rate, and limited available therapies. With the development of high-throughput sequencing technology, the genomic and transcriptomic features of GBM have been fully characterized. Therefore, our study aimed to identify its underlying genetic mechanisms, thus facilitating the development of novel therapies for GBM.

Dynamic Change of Gut Microbiota During Porcine Epidemic Diarrhea Virus Infection in Suckling Piglets.

Porcine epidemic diarrhea (PED) is a disease that has a devastating effect on livestock. Currently, most studies are focused on comparing gut microbiota of healthy piglets and piglets with PED, resulting in gut microbial populations related to dynamic change in diarrheal piglets being poorly understood. The current study analyzed the characteristics of gut microbiota in porcine epidemic diarrhea virus (PEDV)-infected piglets during the suckling transition stage.

Comparison of Oropharyngeal Microbiota in Healthy Piglets and Piglets With Respiratory Disease.

Porcine respiratory disease (PRD) is responsible for severe economic losses in the swine industry worldwide. Our objective was to characterize the oropharyngeal microbiota of suckling piglets and compare the microbiota of healthy piglets and piglets with PRD. Oropharyngeal swabs were collected from healthy (Healthy_A, = 6; Healthy_B, = 4) and diseased (PRD_A, = 18; PRD_B, = 5) piglets at 2-3 weeks of age from two swine farms in Guangdong province, China.

Highly Fluorescent Polyimide Covalent Organic Nanosheets as Sensing Probes for the Detection of 2,4,6-Trinitrophenol.

A new fluorescent polyimide covalent organic framework (PI-COF) has been successfully synthesized through solvothermal route using tetra(4-aminophenyl) porphyrin and perylenetracarboxylic dianhydride, which possesses porous crystalline and excellent thermal stability (>500 °C). Furthermore, few-layered PI covalent organic nanosheets (PI-CONs) can be easily obtained from the fluorescent PI-COF through a facile liquid phase exfoliation approach, which were confirmed by atomic force microscopy and transmission electron microscopy analysis. It is interesting that the fluorescent intensity of PI-CONs is obviously enhanced relative to that of PI-COF.

Validation of measured microwave absorption and temperature change for development of a single-mode-type microwave heating thermogravimetry apparatus.

The temperature distribution, microwave absorption efficiency, and dielectric properties of a copper (ii) oxide (CuO) pellet heated by microwave irradiation were investigated for use in developing a single-mode-type microwave heating thermogravimetry apparatus. The validity of the apparatus was confirmed by comparing the measured data with the results of numerical simulations. The dielectric properties and error margins of other parameters estimated using the apparatus were also examined.