A transient expression tool box for anthocyanin biosynthesis in Nicotiana benthamiana.

Transient expression in Nicotiana benthamiana offers a robust platform for the rapid production of complex secondary metabolites. It has proven highly effective in helping identify genes associated with pathways responsible for synthesizing various valuable natural compounds. While this approach has seen considerable success, it has yet to be applied to uncovering genes involved in anthocyanin biosynthetic pathways.

Phytochemical Characterization of Water Avens ( L.) Extracts: Structure Assignment and Biological Activity of the Major Phenolic Constituents.

Water avens ( L.) is a common plant widely spread in Europe and North America. It is rich in biologically active natural products, some of which are promising as prospective pharmaceuticals.

How Does LC/MS Compare to UV in Coffee Authentication and Determination of Antioxidant Effects? Brazilian and Middle Eastern Coffee as Case Studies.

Coffee is a popular beverage owing to its unique flavor and diverse health benefits. The current study aimed at investigating the antioxidant activity, in relation to the phytochemical composition, of authenticated Brazilian green and roasted and , along with 15 commercial specimens collected from the Middle East. Ultra-high-performance liquid chromatography coupled to high-resolution mass spectrometry (UHPLC-ESI-HRMS) and UV spectrometry were employed for profiling and fingerprinting, respectively.

Anthelmintic and antimicrobial activities of three new depsides and ten known depsides and phenols from Indonesian lichen: Ach.

An extensive phytochemical study of a foliose lichen from Indonesia, , resulted in the successful isolation of 13 phenol and depside derivatives (-) including the previously unreported depsides 3'-hydroxyl-5'-pentylphenyl 2,4-dihydroxyl-6-methylbenzoate (), 3'-hydroxyl-5'-propylphenyl 2,4-dihydroxyl-6-methylbenzoate () and 3'-hydroxyl-5'-methylphenyl 2-hydroxyl-4-methoxyl-6-propylbenzoate (). The anti-infective activity of isolated compounds was evaluated against the gram-negative bacterium and the nematode . 2,4-Dihydroxyl-6-pentylbenzoate () and lecanoric acid () induced growth inhibition of with inhibition values of 49% and 100% at a concentration of 100 µM, respectively.

HPTLC-DESI-HRMS-Based Profiling of Anthraquinones in Complex Mixtures-A Proof-of-Concept Study Using Crude Extracts of Chilean Mushrooms.

High-performance thin-layer chromatography (HPTLC) coupled with negative ion desorption electrospray ionization high-resolution mass spectrometry (DESI-HRMS) was used for the analysis of anthraquinones in complex crude extracts of Chilean dermocyboid Cortinarii. For this proof-of-concept study, the known anthraquinones emodin, physcion, endocrocin, dermolutein, hypericin, and skyrin were identified by their elemental composition. HRMS also allowed the differentiation of the investigated anthraquinones from accompanying compounds with the same nominal mass in the crude extracts.

The unusual fragmentation of long-chain feruloyl esters under negative ion electrospray conditions.

Long-chain ferulic acid esters, such as eicosyl ferulate (1), show a complex and analytically valuable fragmentation behavior under negative ion electrospay collision-induced dissociation ((-)-ESI-CID) mass spectrometry, as studied by use of a high-resolution (Orbitrap) mass spectrometer. In a strong contrast to the very simple fragmentation of the [M + H] ion, which is discussed briefly, the deprotonated molecule, [M - H] , exhibits a rich secondary fragmentation chemistry. It first loses a methyl radical (MS ) and the ortho-quinoid [M - H - Me] radical anion thus formed then dissociates by loss of an extended series of neutral radicals, C H (n = 0-16) from the long alkyl chain, in competition with the expulsion of CO and CO (MS ).

Rhodiola rosea root extract has antipsychotic-like effects in rodent models of sensorimotor gating.

Unlabelled: ETHNOPHARMACOLOGICAL RELEVANCE: The plant arctic root (Rhodiola rosea, L.) is growing in northern regions of Europe, Asia and North America. Extracts of R.

Phytochemical Profiles and Antimicrobial Activities of Allium cepa Red cv. and A. sativum Subjected to Different Drying Methods: A Comparative MS-Based Metabolomics.

Plants of the genus produce sulphur compounds that give them a characteristic (alliaceous) flavour and mediate for their medicinal use. In this study, the chemical composition and antimicrobial properties of red cv. and in the context of three different drying processes were assessed using metabolomics.

A multicomponent macrocyclization strategy to natural product-like cyclic lipopeptides: synthesis and anticancer evaluation of surfactin and mycosubtilin analogues.

A multicomponent macrocyclization strategy towards cyclic lipopeptides is described. The approach relies on the utilization of the Ugi and Passerini multicomponent reactions for the cyclization of peptides and oxo-peptides, and here it is employed for the construction of a small library of analogues of the natural products mycosubtilin and surfactin A. A key feature of this method is the simultaneous incorporation of either one or two exocyclic lipid tails along with the macrocyclic ring closure, which is only possible due to the multicomponent nature of the macrocyclization step.

Tulasporins A-D, 19-Residue Peptaibols from the Mycoparasitic Fungus Sepedonium tulasneanum.

Four new 19-residue peptaibols, named tulasporins A-D (1-4), were isolated from the semi-solid cultures of Sepedonium tulasneanum. Their structures were elucidated on the basis of extensive ESI-HRMS(n) fragmentation studies as well as (1)H NMR spectroscopic analyses. Interestingly, the structures of tulasporins A-D (1-4) resemble those of chrysospermins isolated earlier from cultures of S.

Solution- and Solid-Phase Macrocyclization of Peptides by the Ugi-Smiles Multicomponent Reaction: Synthesis of N-Aryl-Bridged Cyclic Lipopeptides.

A new multicomponent methodology for the solution- and solid-phase macrocyclization of peptides is described. The approach comprises the utilization of the Ugi-Smiles reaction for the cyclization of 3-nitrotyrosine-containing peptides either by the N-terminus or the lysine side-chain amino groups. Both the on-resin and solution cyclizations took place with good to excellent efficiency in the presence of an aldehyde and a lipidic isocyanide, while the use of paraformaldehyde required an aminocatalysis-mediated imine formation prior to the on-resin Ugi-Smiles ring closure.

Chilenopeptins A and B, Peptaibols from the Chilean Sepedonium aff. chalcipori KSH 883.

The Chilean Sepedonium aff. chalcipori strain KSH 883, isolated from the endemic Boletus loyo Philippi, was studied in a polythetic approach based on chemical, molecular, and biological data. A taxonomic study of the strain using molecular data of the ITS, EF1-α, and RPB2 barcoding genes confirmed the position of the isolated strain within the S.