Publications by authors named "AnneCaroline Jousset"
Background: Once integrated in the genome of infected cells, HIV-1 provirus is transcribed by the cellular transcription machinery. This process is regulated by both viral and cellular factors, which are necessary for an efficient viral replication as well as for the setting up of viral latency, leading to a repressed transcription of the integrated provirus.
Results: In this study, we examined the role of two parameters in HIV-1 LTR promoter activity.
RNA molecules are key players in a variety of biological events, and this is particularly true for viral RNAs. To better understand the replication of those pathogens and try to block them, special attention has been paid to the structure of their RNAs. Methods to probe RNA structures have been developed since the 1960s; even if they have evolved over the years, they are still in use today and provide useful information on the folding of RNA molecules, including viral RNAs.
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- * The study introduces in cell Mutational Interference Mapping Experiment (in cell MIME), which allows researchers to identify RNA regulatory functions at a single nucleotide level in their natural environment by mutating RNA, assessing its functionality, and using sequencing techniques.
- * Using in cell MIME, the researchers pinpointed key RNA motifs in HIV-1's genomic RNA that are vital for virus replication, revealing a specific polyadenylation motif that regulates both RNA production and viral packaging, despite its negative impact on RNA production.