Publications by authors named "Anne-Xander van der Stel"
Article Synopsis
- The diagnosis of bone and joint infections (BJI) often depends on slow microbiological cultures, leading researchers to explore faster molecular methods like IS-pro, which can identify bacteria quickly.
- The IS-pro test can provide results in just 4 hours and detects bacterial species while also measuring human DNA to indicate leukocyte levels.
- In a study with 591 synovial fluid samples, IS-pro demonstrated a high agreement with culture methods in identifying bacteria and showed promising results for improving detection rates in diagnosing infections.
Free L-tryptophan (L-Trp) stalls ribosomes engaged in the synthesis of TnaC, a leader peptide controlling the expression of the Escherichia coli tryptophanase operon. Despite extensive characterization, the molecular mechanism underlying the recognition and response to L-Trp by the TnaC-ribosome complex remains unknown. Here, we use a combined biochemical and structural approach to characterize a TnaC variant (R23F) with greatly enhanced sensitivity for L-Trp.
View Article and Find Full Text PDFThe Campylobacterota, previously known as Epsilonproteobacteria, are a large group of Gram-negative mainly, spiral-shaped motile bacteria. Some members like the spp. are free-living, while others such as spp.
View Article and Find Full Text PDFBacteria have evolved different mechanisms to catabolize carbon sources from nutrient mixtures. They first consume their preferred carbon source, before others are used. Regulatory mechanisms adapt the metabolism accordingly to maximize growth and to outcompete other organisms.
View Article and Find Full Text PDFThe generation of a membrane potential (Δψ), the major constituent of the proton motive force (pmf), is crucial for ATP synthesis, transport of nutrients and flagellar rotation. Campylobacter jejuni harbors a branched electron transport chain, enabling respiration with different electron donors and acceptors. Here, we demonstrate that a relatively high Δψ is only generated in the presence of either formate as electron donor or oxygen as electron acceptor, in combination with an acceptor/donor respectively.
View Article and Find Full Text PDFThe highly conserved enzyme γ-glutamyltranspeptidase (GGT) plays an important role in metabolism of glutathione and glutamine. Yet, the regulation of ggt transcription in prokaryotes is poorly understood. In the human pathogen Campylobacter jejuni, GGT is important as it contributes to persistent colonization of the gut.
View Article and Find Full Text PDFArticle Synopsis
- Campylobacter jejuni, a pathogen found in warm-blooded animals, relies on alternative electron acceptors like nitrate and fumarate due to low oxygen in the gastrointestinal tract.
- The RacRS two-component system regulates the production, transport, and reduction of fumarate based on available electron acceptors, affecting C. jejuni's respiration process.
- The study reveals that phosphorylated RacR represses genes related to fumarate utilization, showcasing a unique regulatory mechanism that supports the survival of this pathogen.