Publications by authors named "Anne Wienand"
Article Synopsis
- DiDBiT is a new proteomic technique that enhances the detection of newly synthesized proteins by 20 times compared to traditional methods.
- The new method, DiDBiT-TMT, allows for the quantification of these proteins across multiple conditions and replicates in a single experiment.
- Using DiDBiT-TMT on brain slices, researchers found distinct differences in newly synthesized proteins following different treatments, suggesting unique molecular processes at play in response to chemical long-term potentiation and norepinephrine.
Heterozygous mutations in six transfer RNA (tRNA) synthetase genes cause Charcot-Marie-Tooth (CMT) peripheral neuropathy. CMT mutant tRNA synthetases inhibit protein synthesis by an unknown mechanism. We found that CMT mutant glycyl-tRNA synthetases bound tRNA but failed to release it, resulting in tRNA sequestration.
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