Publications by authors named "Anne Sophie Gille"
Background: A growing number of centers worldwide are preserving testicular tissue (TT) of young boys at risk of fertility loss to preserve their fertility. Data in this regard are scarce and experience sharing is essential to the optimization of the process.
Objectives: This report of our 10-year activity of pediatric fertility preservation (FP) has the objective to (1) improve knowledge regarding the feasibility, acceptability, safety, and potential usefulness of the procedure; (2) analyze the impact of chemotherapy on spermatogonia in the cryopreserved TT.
Male infertility is responsible for approximately half of all cases of reproductive issues. Spermatogenesis originates in a small pool of spermatogonial stem cells (SSCs), which are of interest for therapy of infertility but remain not well defined in humans. Using multiparametric analysis of the side population (SP) phenotype and the α-6 integrin, THY1, and β-2 microglobulin cell markers, we identified a population of human primitive undifferentiated spermatogonia with the phenotype β-2 microglobulin (β-2M)SPα-6THY1, which is highly enriched in stem cells.
View Article and Find Full Text PDFFanconi anemia (FA) is a rare human genetic disorder characterized by bone marrow failure, predisposition to cancer and developmental defects including hypogonadism. Reproductive defects leading to germ cell aplasia are the most consistent phenotypes seen in FA mouse models. We examined the role of the nuclear FA core complex gene Fancg in the development of primordial germ cells (PGCs), the embryonic precursors of adult gametes, during fetal development.
View Article and Find Full Text PDFOngoing progress in genomic technologies offers exciting tools that can help to resolve transcriptome and genome-wide DNA modifications at single-cell resolution. These methods can be used to characterize individual cells within complex tissue organizations and to highlight various molecular interactions. Here, we will discuss recent advances in the definition of spermatogonial stem cells (SSC) and their progenitors in humans using the single-cell transcriptome sequencing (scRNAseq) approach.
View Article and Find Full Text PDFArticle Synopsis
- The study examines the effectiveness of the revised Alpha/ESHRE guidelines from 2017 on managing oocytes containing aggregates of smooth endoplasmic reticulum (SERa) during in-vitro fertilization (IVF).
- It suggests that instead of relying on a case-by-case approach, practitioners should fertilize both SERa+ and SERa- oocytes, while giving priority to embryos from SERa- oocytes due to uncertainties about outcomes.
- The review analyzed 726 studies, narrowing it down to 21 that met its criteria, revealing no strong consensus on the negative impact of SERa on embryological and neonatal outcomes.
Sunitinib Prior to Planned Nephrectomy in Metastatic Renal Cell Carcinoma: Angiogenesis Biomarkers Predict Clinical Outcome in the Prospective Phase II PREINSUT Trial.
Clin Cancer Res
November 2018
Article Synopsis
- The PREINSUT study explored how certain biological markers could predict patients' responses to sunitinib treatment before surgery in those with metastatic renal cell carcinoma (mRCC).
- Conducted in France, the trial involved 32 treatment-naïve patients who received two cycles of sunitinib, aiming to assess both primary tumor size change and potential survival benefits.
- Key findings suggested that specific baseline and treatment-related biomarkers like SDF-1, VEGF, and sVEGFR were linked to tumor response and overall survival, indicating their potential role in guiding antiangiogenic therapy.