Publications by authors named "Anne Silva-Barbosa"
Vascularization plays a critical role in organ maturation and cell-type development. Drug discovery, organ mimicry, and ultimately transplantation hinge on achieving robust vascularization of in vitro engineered organs. Here, focusing on human kidney organoids, we overcame this hurdle by combining a human induced pluripotent stem cell (iPSC) line containing an inducible ETS translocation variant 2 (ETV2) (a transcription factor playing a role in endothelial cell development) that directs endothelial differentiation in vitro, with a non-transgenic iPSC line in suspension organoid culture.
View Article and Find Full Text PDFAcute kidney injury (AKI) is extremely prevalent among hospitalizations and presents a significant risk for the development of chronic kidney disease and increased mortality. Ischemia caused by shock, trauma, and transplant are common causes of AKI. To attenuate ischemic AKI therapeutically, we need a better understanding of the physiological and cellular mechanisms underlying damage.
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- Dicarboxylic fatty acids like dodecanedioic acid (DC12) are produced in the liver and kidney, but their dietary effects are unexplored.
- In a study, mice were fed DC12 at 20% of their daily caloric intake for nine weeks, resulting in increased metabolic rate, reduced body fat, and improved glucose tolerance.
- The research shows that DC12 was metabolized in various tissues and may be beneficial for addressing obesity and metabolic disorders.
Article Synopsis
- - The study finds that octanedioic acid (DC 8) can shield mice from kidney damage due to injuries like ischemia-reperfusion and cisplatin chemotherapy by enhancing the breakdown of fats through peroxisomal activity without harming mitochondrial function.
- - Mice fed a diet with dicarboxylic acids received significant protection from markers indicating acute kidney injury (AKI), with DC 8 preserving critical cellular components and promoting fat oxidation.
- - These promising results point toward DC 8 as a potential treatment option for patients at risk for kidney damage, suggesting both safety and effectiveness in protecting renal health.
Acute kidney injury (AKI) manifests as a major health concern, particularly for the elderly. Understanding AKI-related proteome changes is critical for prevention and development of novel therapeutics to recover kidney function and to mitigate the susceptibility for recurrent AKI or development of chronic kidney disease. In this study, mouse kidneys were subjected to ischemia-reperfusion injury, and the contralateral kidneys remained uninjured to enable comparison and assess injury-induced changes in the kidney proteome.
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- Vascularization is super important for organs to grow and for cells to develop properly, especially in making organs like kidneys for medical use.
- Scientists created human kidney organoids that have good blood vessel structures by mixing different types of stem cells in a special culture.
- These organoids not only have better blood flow but also show improved development of kidney cells, making this research a big step towards using lab-grown organs in real medical treatments.
Article Synopsis
- Acute kidney injury (AKI) is a significant health issue, especially in elderly populations, and research into its proteome changes is vital for new treatments.
- The study analyzed mouse kidneys affected by ischemia-reperfusion injury using advanced mass spectrometry for in-depth protein identification and quantification.
- Results showed that over half of the proteins in the injured kidneys changed significantly, with a notable decline in proteins related to energy production, pointing to a complete remodeling of the kidney proteome in response to AKI.
Background: Studies have suggested that estrogens may protect mice from AKI. Estrogen sulfotransferase (, or EST) plays an important role in estrogen homeostasis by sulfonating and deactivating estrogens, but studies on the role of in AKI are lacking.
Methods: We used the renal ischemia-reperfusion model to investigate the role of in AKI.