Oxidative deoxyribonucleic acid damage in sperm has a negative impact on clinical pregnancy rate in intrauterine insemination but not intracytoplasmic sperm injection cycles.

Objective: To determine the level of DNA damage, both fragmentation and oxidative, in the sperm population used for intrauterine insemination (IUI) and intracytoplasmic sperm injection (ICSI) and its impact on fertilization and clinical pregnancy rates.

Design: A prospective clinical study.

Setting: A tertiary care fertility clinic.

The effect of repeated freezing and thawing on human sperm DNA fragmentation.

Objective: To investigate the effects of repeated freezing and thawing on human sperm motility, vitality, and DNA integrity.

Design: A prospective clinical study.

Setting: Tertiary care fertility clinic.

The DNA integrity of cryopreserved spermatozoa separated for use in assisted reproductive technology is unaffected by the type of cryoprotectant used but is related to the DNA integrity of the fresh separated preparation.

Objective: To investigate and compare seven different commercially available cryoprotectant media in terms of the DNA integrity of spermatozoa recovered after cryopreservation and separation using density gradient centrifugation (DGC).

Design: A prospective clinical study.

Setting: Tertiary care fertility clinic.