Publications by authors named "Anne Marie Roch"
Article Synopsis
- The addition of 4-methylthio-2-oxobutanoate (MTOB) helps methionine-dependent cancer cells grow without methionine and transition state inhibitors can selectively target these cancer cells for inhibition.
- The transition state analogue L-methionine ethyl ester pyridoxal (MEEP) and the amine donor analogue D-aspartate beta hydroxamate (D-AH) effectively inhibit the MTOB transaminase enzyme in cancer cells, promoting apoptosis without affecting normal cells.
- MEEP shows 20 times greater efficacy than D-AH in inducing cancer cell death, highlighting MTOB transaminase as a promising target for developing selective anticancer therapies.
The 81D1C2 monoclonal antibody (Mab) directed against the Nepsilon-(gamma-L-glutamyl)-L-lysine isopeptide was found to cross-react on Enzyme Immuno Assay (EIA) with acylated lysines. Using a differential screening EIA procedure, a new Mab 81D4 was selected, which did not cross-react with acylated lysines but exhibited strong reactivity with Nepsilon-(gamma-L-glutamyl)-L-lysine formed by covalently coupling the gamma-carboxyl of NalphaCBZ OtBu glutamic acid to epsilon-NH2 derivatized microtiter plates. When Nepsilon-(gamma-L-glutamyl)-L-lysine isopeptides were generated on gamma-carboxyl derivatized plates, only lysine isopeptides with blocked alpha-amines were reactive, regardless of whether the bond formed by the amine blocking agent was a carbamate with carbobenzyloxychloride or an amide with acetic anhydride.
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