The impact of SWI/SNF and NuRD inactivation on gene expression is tightly coupled with levels of RNA polymerase II occupancy at promoters.

SWI/SNF and NuRD are protein complexes that antagonistically regulate DNA accessibility. However, repression of their activities often leads to unanticipated changes in target gene expression (paradoxical), highlighting our incomplete understanding of their activities. Here we show that SWI/SNF and NuRD are in a tug-of-war to regulate PRC2 occupancy at lowly expressed and bivalent genes in mouse embryonic stem cells (mESCs).

H3K9 dimethylation safeguards cancer cells against activation of the interferon pathway.

Activation of interferon genes constitutes an important anticancer pathway able to restrict proliferation of cancer cells. Here, we demonstrate that the H3K9me3 histone methyltransferase (HMT) suppressor of variegation 3-9 homolog 1 (SUV39H1) is required for the proliferation of acute myeloid leukemia (AML) and find that its loss leads to activation of the interferon pathway. Mechanistically, we show that this occurs via destabilization of a complex composed of SUV39H1 and the two H3K9me2 HMTs, G9A and GLP.

Phosphorus recovery by ion exchange in a solid carbonate: modeling of the process.

Phosphorus (P) is a nutrient for plant growth but also a pollutant in water bodies causing eutrophication. The source of P is mainly human and animal wastewater and runoffs from different land uses. The objective of the present study is to evaluate P removal and recovery processes by ion exchange (IE) with solid carbonate (SC) in biodigestor-treated swine effluent (BTSE) using hydrogeochemical modeling.

Effect of mono and divalent salts on the conformation and composition of a humic acid and on atrazine adsorption.

We investigated the effects of sodium and calcium chlorides on the conformation and composition of a purified Aldrich humic acid (PAHA), as well as on the adsorption of atrazine. The PAHA was treated with 1, 10, and 100 mM NaCl, CaCl, or a mixture of NaCl and CaCl (molar ratio 5:1) at pH 7.5 and 8.

Nutrient loads in the river mouth of the Río Verde basin in Jalisco, Mexico: how to prevent eutrophication in the future reservoir?

Since nutrients are emitted and mobilized in river basins, causing eutrophication of water bodies, it is important to reduce such emissions and subsequent nutrient loads. Due to processes of attenuation, nutrient loads are reduced during their mobilization in river basins. At the mouth of the Río Verde basin in western Mexico, the El Purgatorio dam is being constructed to supply water to the metropolitan area of the second most populated city in the country, Guadalajara.

Internal phosphorus load in a Mexican reservoir through sediment speciation analysis.

Since the sequential extraction of phosphorus (P) in sediment makes it possible to determine the P potentially available for release, in this paper, we evaluate the fractions of P in sediment profiles from Valle de Bravo reservoir, a eutrophic lake in central Mexico to determine the contributions of each fraction to the internal P load (IPL). The P fractionation scheme employs sequential extractions of sediment with O-free water (MilliQ), bicarbonate-dithionite (BD), sodium hydroxide (NaOH), hydrochloric acid (HCl), and potassium persulfate (KSO-) to obtain five P fractions. A monitoring of redox potential (Eh), pH, and total phosphorus (TP) in the bottom water of the reservoir indicated variations of these parameters during the year, observing that as Eh decreased, the P concentration increased, it was also observed that when increasing pH, P concentration also increased.

Internal phosphorus load in a Mexican reservoir: forecast and validation.

To determine the internal phosphorus load (IPL) as a function of redox potential (Eh) in a Mexican reservoir, the results from a phosphorus (P) release experiment were extrapolated to temporal and spatial variations of Eh in sediments, and an IPL-Eh of 24.2 ± 2.5 t/yr was obtained.

Unpacking University-Community Partnerships to Advance Scholarship of Practice.

Today, more than ever, occupational therapists are engaged in close partnerships with community organizations and community settings such as service agencies, refugee and immigrant enclaves, and faith-based organizations, to name a few, for the purpose of engaging in scholarship of practice. However, we know little about the views of community partners regarding the development and sustainability of university-community partnerships. The purpose of this article is twofold: First, we will describe a pilot study in which we gathered qualitative data from community partners engaged in scholarship of practice with faculty and students, regarding their views about benefits of partnerships, challenges, and characteristics of sustainable partnerships.

A chi-square goodness-of-fit test for autoregressive logistic regression models with applications to patient screening.

We propose a chi-square goodness-of-fit test for autoregressive logistic regression models. General guidelines for a two-dimensional binning strategy are provided, which make use of two types of maximum likelihood parameter estimates. For smaller sample sizes, a bootstrap p-value procedure is discussed.

Volatility of organic aerosol: evaporation of ammonium sulfate/succinic acid aqueous solution droplets.

Condensation and evaporation modify the properties and effects of atmospheric aerosol particles. We studied the evaporation of aqueous succinic acid and succinic acid/ammonium sulfate droplets to obtain insights on the effect of ammonium sulfate on the gas/particle partitioning of atmospheric organic acids. Droplet evaporation in a laminar flow tube was measured in a Tandem Differential Mobility Analyzer setup.

Observations of mercury wet deposition in Mexico.

We provide a longer-term record of Hg wet deposition at two tropical latitude monitoring sites in Mexico, selected to provide regionally representative data. Weekly wet deposition samples were collected over 2 years, from September 2003 to November 2005. Based on this data set, we discuss the magnitude and seasonal variation of Hg in wet deposition and compare the results to other measurement sites and to several model estimates.

Development and validation of an abridged measure of organizational justice.

In this 3-Study article, the authors present an abridged version of Colquitt's (2001) 20-item measure of organizational justice. In Study 1, using a sample of 278 undergraduate students, they applied a systematic scale-reduction technique to determine which items should remain in the abridged scale. In Study 2 and 3, using two separate field samples (N = 173, N = 517; respectively) they provide construct, convergent, and discriminant validity evidence in support of the abridged scale.

Bridging theory and practice: occupational justice and service learning.

Objective: This article presents a service learning pedagogy whereby students develop the skills of an evidence-based practice scholar committed to occupational justice as a means to transform occupational therapy practice, their clients, themselves and the world.

Participants: Fifty-four fourth year occupational therapy students in a five-year master's program.

Methods: During a two-semester course in clinical reasoning, occupational therapy students participate in service learning with marginalized and vulnerable populations.

Anchorless surface associated glycolytic enzymes from Lactobacillus plantarum 299v bind to epithelial cells and extracellular matrix proteins.

An important criterion for the selection of a probiotic bacterial strain is its ability to adhere to the mucosal surface. Adhesion is usually mediated by proteins or other components located on the outer cell surface of the bacterium. In the present study we characterized the adhesive properties of two classical intracellular enzymes glyceraldehyde 3-phosphate dehydrogenase (GAPDH) and enolase (ENO) isolated from the outer cell surface of the probiotic bacterium Lactobacillus plantarum 299v.

In vivo detection of cortical optical changes associated with seizure activity with optical coherence tomography.

The most common technology for seizure detection is with electroencephalography (EEG), which has low spatial resolution and minimal depth discrimination. Optical techniques using near-infrared (NIR) light have been used to improve upon EEG technology and previous research has suggested that optical changes, specifically changes in near-infrared optical scattering, may precede EEG seizure onset in in vivo models. Optical coherence tomography (OCT) is a high resolution, minimally invasive imaging technique, which can produce depth resolved cross-sectional images.

Mild cognitive impairment: prodromal Alzheimer's disease or something else?

The majority of mild cognitive impairment (MCI) studies use baseline and one follow-up measurement to determine the clinical course of the disorder. This report of MCI clinical course is based on the a statistical evaluation of multiple neurocognitive tests over a 60 month period in elderly normal and MCI cohorts. The data includes serial informant-based measures (Clinical Dementia Rating [CDR]) and a comprehensive battery of neuropsychological tests analyzed by two different regression methods.

Scenarios of metal concentrations in the Arcediano Dam (State of Jalisco, Mexico).

The city of Guadalajara, Jalisco, Mexico has 4.1 million inhabitants and a serious deficit in water supply. Once constructed, the Arcediano Dam will catch waters from the Verde and Santiago rivers, and after treatment will provide water to the city.

Proteomic analysis of cell surface-associated proteins from probiotic Lactobacillus plantarum.

In the present study, we used a proteomic approach to identify surface-associated proteins from the probiotic bacterium Lactobacillus plantarum 299v. Proteins were extracted from the cell surface using a mild wash in phosphate buffer and analysed by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. Gel bands were excised and in-gel digested with trypsin.

Oat (Avena sativa) seed extract as an antifungal food preservative through the catalytic activity of a highly abundant class I chitinase.

Extracts from different higher plants were screened for the ability to inhibit the growth of Penicillium roqueforti, a major contaminating species in industrial food processing. Oat (Avena sativa) seed extracts exhibited a high degree of antifungal activity and could be used directly on rye bread to prevent the formation of P. roqueforti colonies.

Food products as vehicles for n-3 fatty acid supplementation.

Purpose: An n-3 polyunsaturated fatty acid (PUFA) supplement was incorporated into three food products previously determined to be preferred by cancer patients, and overall acceptability of these foods was evaluated.

Methods: Preliminary testing was performed; an internal panel determined initial acceptability of foods with the supplement added. Taste panel evaluations were held at the Cross Cancer Institute in Edmonton, Alberta.

Wavelength dependence of light-induced lipid oxidation and naturally occurring photosensitizers in cheese.

Degradation of the potential photosensitizers, riboflavin, chlorophyll, and porphyrin, in Danbo cheese by monochromatic light of wavelength 366, 436, or 546 nm was studied. Three cheeses were investigated, two conventional (16% fat and 25% fat) and one "organic" (25% fat). The effect of illumination was measured by fluorescence spectroscopy and analyzed using multiway and multivariate data analysis.

Mineralization of atrazine in agricultural soil: inhibition by nitrogen.

Microbial mineralization of atrazine was characterized in soils and liquid media in the presence of nitrogen fertilizer concentrations representing typical field applications. The mineralization of atrazine in soils varied between 6 and 99% after 18 d of incubation. Half-lives of between 0.

Service learning: meaningful, community-centered professional skill development for occupational therapy students.

The profession of Occupational Therapy advocates for new and emerging areas of practice that more fully embrace our moral responsibility to address significant social injustices that exist in our communities (Kronenberg, Algado, & Pollard, 2005). The service-learning pedagogy is impacted by the philosophical and theoretical influences of John Dewey, the mission and purpose of American higher education including Boyer's (1994) call for an engaged citizenry, and the social vision of occupational therapy. The pedagogy of service learning provides a natural context for students to experience community practice while contributing to reducing existing social injustices.