Publications by authors named "Anne Katrine W Simonsen"
Article Synopsis
- Investigated the role of human topoisomerase 1 (TOP1) in regulating G-quadruplex (G4) formation in the Pu27 region of the MYC promoter, where G4 formation inhibits promoter activity.
- Found that downregulating TOP1 increased transcription from a wildtype Pu27 promoter, but this effect was absent if G4 formation was disrupted by mutations; similar effects were observed with another G4 forming element, WT21.
- Demonstrated for the first time that TOP1 can induce G4 formation in double-stranded DNA in vitro and that it selectively interacts with the wildtype Pu27 sequence, highlighting its significant role in G4 dynamics within cells.
Replication-blocking DNA lesions are particularly toxic to proliferating cells because they can lead to chromosome mis-segregation if not repaired prior to mitosis. In this study, we report that ZGRF1 null cells accumulate chromosome aberrations following replication perturbation and show sensitivity to two potent replication-blocking anticancer drugs: mitomycin C and camptothecin. Moreover, ZGRF1 null cells are defective in catalyzing DNA damage-induced sister chromatid exchange despite accumulating excessive FANCD2, RAD51, and γ-H2AX foci upon induction of interstrand DNA crosslinks.
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