Antiviral effect of cationic compounds on bacteriophages.

The antiviral activity of several cationic compounds - cetyltrimethylammonium bromide (CTAB), chitosan, nisin, and lysozyme - was investigated on the bacteriophage c2 (DNA head and non-contractile tail) infecting Lactococcus strains and the bacteriophage MS2 (F-specific RNA) infecting E. coli. Firstly, these activities were evaluated in a phosphate buffer pH 7 - 10 mM.

Direct Quantitative Detection and Identification of Lactococcal Bacteriophages from Milk and Whey by Real-Time PCR: Application for the Detection of Lactococcal Bacteriophages in Goat's Raw Milk Whey in France.

The presence of Lactococcus bacteriophages in milk can partly or completely inhibit milk fermentation. To prevent the problems associated with the bacteriophages, the real-time PCR was developed in this study for direct detection from whey and milk of three main groups of Lactococcus bacteriophages, c2, 936, and P335. The optimization of DNA extraction protocol from complex matrices such as whey and milk was optimized allowed the amplification of PCR without any matrix and nontarget contaminant interference.

Description of a French natural wheat sourdough over 10 consecutive days focussing on the lactobacilli present in the microbiota.

Sourdoughs are complex ecosystems which are widely used to produce baked goods. This study aimed to provide a dynamic description of an industrial French sourdough ecosystem over 10 consecutive days. The sourdough was obtained from a natural fermentation that has been propagated over several years by the traditional backslopping method.

Comparative study of culture media used for sourdough lactobacilli.

Eleven culture media were analysed to compare their exhaustiveness for the quantification of lactobacilli type I sourdoughs. The media tested were MRS, maltose modified MRS, MRS5, SDB, SFM, MRS "Vogel", Rogosa as elective media, and FH, OH, LAMVAB, KCA as selective media. Six broth media were also tested for colony subculture.