Super-resolved coherent anti-Stokes Raman scattering microscopy by coherent image scanning.

We present super-resolved coherent anti-Stokes Raman scattering (CARS) microscopy by implementing phase-resolved image scanning microscopy, achieving up to two-fold resolution increase as compared with a conventional CARS microscope. Phase-sensitivity is required for the standard pixel-reassignment procedure since the scattered field is coherent, thus the point-spread function is well-defined only for the field amplitude. We resolve the complex field by a simple add-on to the CARS setup enabling inline interferometry.

Spatio-temporal coherence of circadian clocks and temporal control of differentiation in filaments.

Circadian clock arrays in multicellular filaments of the heterocyst-forming cyanobacterium sp. strain PCC 7120 display remarkable spatio-temporal coherence under nitrogen-replete conditions. To shed light on the interplay between circadian clocks and the formation of developmental patterns, we followed the expression of a clock-controlled gene under nitrogen deprivation, at the level of individual cells.

Robust, coherent, and synchronized circadian clock-controlled oscillations along filaments.

Circadian clocks display remarkable reliability despite significant stochasticity in biomolecular reactions. We study the dynamics of a circadian clock-controlled gene at the individual cell level in sp. PCC 7120, a multicellular filamentous cyanobacterium.