Multi-functional regulation of 4E-BP gene expression by the Ccr4-Not complex.

The mechanistic target of rapamycin (mTOR) signaling pathway is highly conserved from yeast to humans. It senses various environmental cues to regulate cellular growth and homeostasis. Deregulation of the pathway has been implicated in many pathological conditions including cancer.

Use of anionic denaturing detergents to purify insoluble proteins after overexpression.

Background: Many proteins form insoluble protein aggregates, called "inclusion bodies", when overexpressed in E. coli. This is the biggest obstacle in biotechnology.