Complex of Defense Polypeptides of Wheatgrass () Associated with Plant Immunity to Biotic and Abiotic Stress Factors.

Plant defense polypeptides play a crucial role in providing plants with constitutive immunity against various biotic and abiotic stressors. In this study, we explored a complex of proteins from wheatgrass () spikelets to estimate their role in the plant's tolerance to various environmental factors. The current research shows that in vitro protein extracts from spikelets possess antifungal activity against certain species, which are specific cereal pathogens, at concentrations of 1-2 mg/mL.

Retention mechanisms of dipeptides on superficially porous particle vancomycin- and teicoplanin-based chiral stationary phases.

Chromatographic behavior of new chiral stationary phases (CSPs) Chiral-T and Chiral-V with teicoplanin and vancomycin antibiotics grafted onto superficially porous silica particles was studied in relation to dipeptide (DP) stereoisomers. The unbuffered water-methanol solutions were used as mobile phases (MPs). The effects of physical properties and molecular structure of analytes and selectors on retention and separation of DP stereoisomers are discussed herein.

Recombinant Fusion Protein Containing Plant Nigellothionin Regulates the Growth of Food-Spoiling Fungus ().

This study aimed to obtain a recombinant chimeric protein named trx-NsW2 via theheterologous expression of the multifunctional antimicrobial peptide nigellothionin from black cumin ( L.) seeds in the system. The protein was purified using a combination of Ni-NTA affinity chromatography and reversed-phase HPLC.

Diversity of Cationic Antimicrobial Peptides in Black Cumin ( L.) Seeds.

Black cumin ( L.) is known to possess a wide variety of antimicrobial peptides belonging to different structural families. Three novel antimicrobial peptides have been isolated from black cumin seeds.

C Fullerene Clusters Stabilize the Biologically Inactive Form of Topotecan.

There is a range of experimental proofs that biologically relevant compounds change their activity in the presence of C fullerene clusters in aqueous solution, which most frequently act as a nanoplatform for drug delivery. Inspired by this evidence, we made an effort to investigate the interaction of fullerene clusters with the antibiotic topotecan (TPT). This study proceeded in three steps, namely, UV/vis titration to confirm complexation and in vitro assays on proliferating and nonproliferating cells to elucidate the role of C fullerene in the putative change in TPT activity.

Rational Design of Plant Hairpin-like Peptide EcAMP1: Structural-Functional Correlations to Reveal Antibacterial and Antifungal Activity.

Plant antimicrobial peptides from the α-hairpinins family (hairpin-like peptides) are known to possess a wide range of biological activities. However, less is known about the structural determinants of their antimicrobial activity. Here, we suggest that spatial structure as well as surface charge and hydrophobicity level contribute to the antimicrobial properties of α-hairpinin EcAMP1 from barnyard grass () seeds.

Exploring Peptaibol's Profile, Antifungal, and Antitumor Activity of Emericellipsin A of Species from Soda and Saline Soils.

Features of the biochemical adaptations of alkaliphilic fungi to exist in extreme environments could promote the production of active antibiotic compounds with the potential to control microorganisms, causing infections associated with health care. Thirty-eight alkaliphilic and alkalitolerant Emericellopsis strains (E. alkalina, E.

Nigellothionins from Black Cumin ( L.) Seeds Demonstrate Strong Antifungal and Cytotoxic Activity.

High-cationic biologically active peptides of the thionins family were isolated from black cumin ( L.) seeds. According to their physicochemical characteristics, they were classified as representatives of the class I thionin subfamily.

Isolation of antimicrobial peptides from different plant sources: Does a general extraction method exist?

Plants are good sources of biologically active compounds with antimicrobial activity, including polypeptides. Antimicrobial peptides (AMPs) represent one of the main barriers of plant innate immunity to environmental stress factors and are attracting much research interest. There are some extraction methods for isolation of AMPs from plant organs based on the type of extractant and initial fractionation stages.

Peptide Extracts from Seven Medicinal Plants Discovered to Inhibit Oomycete a Causative Agent of Potato Late Blight Disease.

We report the inhibitory effect of peptide extracts obtained from seven medicinal plants against a causative agent of late blight disease We find that all the extracts possess inhibitory activity toward the zoospores output, zoosporangium germination, and the development of on potato disc tubers at different quantitative levels. Based on the biological effects detected, an extract of common horsetail () biomass is recognized as the most effective and is selected for further structural analysis. We perform a combination of amino acid analysis and MALDI-TOF mass spectrometry, which reveal the presence of Asn/Asp- and Gln/Glu-rich short peptides with molecular masses in the range of 500-900 Da and not exceeding 1500 Da as the maximum.